Project description:Changes in global gene expression in the epidermis of the Brg1(i)ep-/- mice in comparison to the wild type at E16.5 were analyzed using micro-array technology.
Project description:Changes in global gene expression in the epidermis of the Brg1(i)ep-/- mice in comparison to the wild type at E16.5 were analyzed using micro-array technology. The RNA was purified from the mouse epidermal tissue after laser capture microdisection (LCM) followed by two rounds of linear amplification. The amplified RNA from two Brg1(i)ep-/- animals were pooled together and the amplified RNA from two control animals was also were pooled together.
Project description:Potential Stat3 target genes involved in hair follicle formation were examined using mouse whole-genome microarray analysis. Global gene expression patterns in the epidermis of transgenic mice with keratinocytes expressing a constitutively active Stat3BK5.Stat3C mice (Sano et al., 2005) were compared with the pattern in the epidermis of wild-type mice. Of approximately 130 differentially expressed genes detected by microarray, approximately 70 genes were significantly up-regulated in epidermis by constitutive activation of Stat3. Thirty three of these genes were hair follicle related genes. These genes included trichohyalin, hair keratins, and keratin-associated proteins that are expressed in the hair shaft and inner root sheath as essential structural components for hair growth (Winter et al., 1997). To confirm the microarray results, five genes whose expression was up-regulated in the epidermis of BK5.Stat3C mice were chosen for quantitative RT-PCR analysis. Consistent with the microarray data, the expression of Tchh, Krtap16-8, Krtap6-1, Krt33a, and Krt25d was significantly increased in the epidermis of BK5.Stat3C mice compared to wild-type mice. In addition, Krtap16-1, which was not identified by microarray analysis but is localized on mouse chromosome 16, was highly expressed in the epidermis of BK5.Stat3C mice in comparison to wild-type (Figure 1f).
Project description:Endodermal progenitor cells (EP cells) are derived from human embryonic stem cell(ESC)-derived definitive endoderm (DE) cells. EP cells are cultured in high BMP media and DE cells are in high Activin media. Both cells can be further differentiated to liver, pancreas, etc. We used microarray to detail the global gene expression profile of DE cells and EP cells to delineate the difference of DE cells and EP cells.
Project description:Binding of Brg1, Sall1, H3K4me1, H3K27ac, and RNA Pol2 Ser2 to chromatin was measured by performing ChIP-seq in E16.5 wild type kidney.
