Project description:We sequenced non-ribosomal RNA from a upf1 deletion mutant of S. cerevisiae, and from wild type S. mikatae and S. bayanus, and identified a new class of yeast introns.
Project description:In this work, we report that the knockout mutant in other of this luxR-like genes (named luxR402) in Novosphingobium sp. HR1a; floculated faster than the wild-type when cultures are in repose. Transcriptomic analysis allowed us to determine the LuxR402 regulon; we have identified that the carbohydrate assimilation pathway and TCA cycle are affected in the mutant. Accordingly, the mutant presented poorer growth than the wild-type when growing in different carbon sources. At stationary phase of growth the pili biosynthesis, trehalose utilization and capside-related proteins were affected in the mutant strain. Microscopy assays determined that the mutant strain cultures presented cells aggrupations and that the extracellular matrix is less abundant than in the wild-type cultures.
Project description:Abstract: A sensor histidine kinase of Synechococcus sp. strain PCC7942, designated nblS, was previously identified and shown to be critical for the acclimation of cells to high-light and nutrient limitation conditions and to influence the expression of a number of light-responsive genes. The nblS orthologue in Synechocystis sp. strain PCC6803 is designated dspA (also called hik33). We have generated a dspA null mutant and analyzed global gene expression in both the mutant and wild-type strains under high- and low-light conditions. The mutant is aberrant for the expression of many genes encoding proteins critical for photosynthesis, phosphate and carbon acquisition, and the amelioration of stress conditions. Furthermore, transcripts from a number of genes normally detected only during exposure of wild-type cells to high-light conditions become partially constitutive in the low-light-grown dspA mutant. Other genes for which transcripts decline upon exposure of wild-type cells to high light are already lower in the mutant during growth in low light. These results suggest that DspA may influence gene expression in both a positive and a negative manner and that the dspA mutant behaves as if it were experiencing stress conditions (e.g., high-light exposure) even when maintained at near-optimal growth conditions for wild-type cells. This is discussed with respect to the importance of DspA for regulating the responses of the cell to environmental cues. A strain or line experiment design type assays differences between multiple strains, cultivars, serovars, isolates, lines from organisms of a single species. Keywords: strain_or_line_design