Genomics

Dataset Information

235

Synthetic construct


ABSTRACT: Sub-genomewide shRNAs constructed using an optimized selection algorithm and microRNA backbone provide stronger evidence for follow-up studies

ORGANISM(S): synthetic construct  

PROVIDER: PRJNA263392 | ENA |

REPOSITORIES: ENA

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Publications


The strength of conclusions drawn from RNAi-based studies is heavily influenced by the quality of tools used to elicit knockdown. Prior studies have developed algorithms to design siRNAs. However, to date, no established method has emerged to identify effective shRNAs, which have lower intracellular abundance than transfected siRNAs and undergo additional processing steps. We recently developed a multiplexed assay for identifying potent shRNAs and used this method to generate ∼250,000 shRNA effi  ...[more]

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