Dataset Information


Homo sapiens

ABSTRACT: Genome-Wide Specificity of DNA-Binding, Gene Regulation, and Chromatin Remodeling by TALE- and CRISPR/Cas9-Based Transcription Factors

ORGANISM(S): Homo sapiens  



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Genome-wide specificity of DNA binding, gene regulation, and chromatin remodeling by TALE- and CRISPR/Cas9-based transcriptional activators.

Polstein Lauren R LR   Perez-Pinera Pablo P   Kocak D Dewran DD   Vockley Christopher M CM   Bledsoe Peggy P   Song Lingyun L   Safi Alexias A   Crawford Gregory E GE   Reddy Timothy E TE   Gersbach Charles A CA  

Genome research 20150529 8

Genome engineering technologies based on the CRISPR/Cas9 and TALE systems are enabling new approaches in science and biotechnology. However, the specificity of these tools in complex genomes and the role of chromatin structure in determining DNA binding are not well understood. We analyzed the genome-wide effects of TALE- and CRISPR-based transcriptional activators in human cells using ChIP-seq to assess DNA-binding specificity and RNA-seq to measure the specificity of perturbing the transcripto  ...[more]

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