Project description:This data is a case study done in the context of developing methods for assessing the taxonomic composition of microbial communities using metaproteomics. For this study with analyzed phototrophic biomats from two Soda Lakes in the Canadian Rocky Mountains using metaproteomics. For protein identification we generated a metagenome from which we predicted and annotated the protein sequences used to analyze the metaproteomes. The database is available in this PRIDE submission. Lake1 refers to Goodenough Lake (GEM, 51°19'47.64"N 121°38'28.90"W) and Lake2 referes to Last Chance Lake (LCM, 51°19'39.3" N 121°37'59.3"W).
Project description:Transcriptional Regulation in liver and ovary associated with developmental and reproductive effects in female Zebrafish exposed to natural mixtures of persistent organic pollutants from Lake Mjosa or Lake Losna
Project description:We examined adaptive morphological divergence and epigenetic variation in genetically impoverished asexual populations of a freshwater snail, Potamopyrgus antipodarum from distinct environments. These populations exhibit environment-specific adaptive divergence in shell shape and significant genome wide DNA methylation differences among differentially adapted lake and fast water flow river populations. The epigenetic variation correlated with adaptive phenotypic variation in rapidly adapting asexual animal populations. This provides one of the first examples of environmentally-driven differences in epigenetics that associates with adaptive phenotypic divergence. Overall design: To examine the association between this adaptive divergence and epigenetic differences, we sampled at least 30 individuals from a “river” site characterized by constantly fast water current speeds due to input from underground springs (Ritter Island, Snake River, ID) and from a “lake” site with no water current (Lake Washington, Seattle, WA). Shell spire height and aperture length were measured as an index of adaptive foot size and shell shape differences. On these same samples, we dissected foot tissue from individuals for the analysis of epigenetic variation.