Project description:In this study, small RNAs were isolated from individual donations of eight forensically relevant biological fluids (blood, semen, vaginal fluid, menstrual blood, saliva, urine, feces, and perspiration) and subjected to next generation sequencing using the Illumina® Hi-Seq platform. Sequencing reads were aligned and annotated against miRbase release 21, resulting in a list of miRNAs and their relative expression levels for each sample analyzed. Body fluids with high bacterial loads (vaginal fluid, saliva, and feces) yielded relatively low annotated miRNA counts, likely due to oversaturation of small RNAs from the endogenous bacteria. Both body-fluid specific and potential normalization miRNAs were identified for further analysis as potential body fluid identification tools for each body fluid. 32 samples - 3-5 replicates of each human biological fluid: venous blood, urine, semen (normal and vasectomized), vaginal secretions, menstrual secretions, perspiration, feces, saliva
Project description:We collected adipose tissue specimens during elective surgery (inguinal hernia, cryptorchism). Samples were proceesed for extraction of total RNA, using phenol:chlorophorm extraction. Next generation RNA sequencing were performed. Published article: https://www.mdpi.com/1422-0067/24/23/16706
Project description:As obligate intracellular parasites, viruses rely heavily on their host cells for their replication, and therefore dysregulate several cellular processes for their benefit. In return, host cells activate multiple signaling pathways to limit viral replication and eradicate viruses. The present study explores the complex interplay between viruses and their host cells through next generation RNA sequencing as well as mass spectrometry (SILAC).
Project description:To uncover the host response pathways that are modified by a B. subtilis diet and that may provide the protective effect against α-synuclein aggregation, we performed comparative global transcriptomics analysis using next generation RNA sequencing (RNA-seq). We compared young adult animals fed on three different diets, E. coli OP50 and B. subtilis PXN21 lawns, consisting of both spores and vegetative cells, and a mixture of both bacteria.
Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare the small RNA profile of healthy and myeloma plasma cells derived from non cancer donors and newly diagnosed myeloma patients Methods: Plasma Cell small RNA profiles of 3 healthy plasma cells and 3 newly diagnosed myeloma patients were generated by deep sequencing, using Illumina GAIIx. Conclusions: Our study represents the first detailed analysis of small RNA sequencing in plasma cells. The optimized data analysis workflows reported here should provide a framework for comparative investigations of expression profiles. Our results show that NGS offers a comprehensive quantitative and qualitative evaluation of small RNA content within the healthy and myeloma plasma cells.
