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Bacillus thuringiensis serovar chinensis CT-43 strain:CT43

ABSTRACT: This strain will be used for comparative genome analysis

PROVIDER: PRJNA30163 | ENA |

REPOSITORIES: ENA

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Transcriptomics of Bacillus thuringiensis Strain CT-43.

2013-04-17 | GSE39479 | GEO

Transcriptomics of Bacillus thuringiensis Strain CT-43.

Project description:We investigated the gene expression and metabolic regulatory mechanisms associated with the high-level accumulation of ICPs by performing the transcriptomics analysis of B. thuringiensis strain CT-43, using Illumina high throughout sequencing (RNA-seq) technique. The bacterial cells were collected at the time points of 7 h, 9 h, 13 h and 22 h for the whole-genome transcriptomics, respectively.

2013-04-17 | E-GEOD-39479 | biostudies-arrayexpress

Complete genome sequence of Bacillus thuringiensis subsp. chinensis strain CT-43.

Project description: Not available

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Bacillus thuringiensis serovar chinensis CT-43

Project description:Bacillus thuringiensis serovar chinensis CT-43 Transcriptome or Gene expression

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Bacillus thuringiensis serovar chinensis CT-43 strain:CT43

Project description:Bacillus thuringiensis CT43 genome sequencing

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MS identification of the Cry proteins in Bacillus thuringiensis CT-43

Project description:In Bacillus thuringiensis CT-43, five insecticidal crystal proteins (ICPs, Cry protein) are encoded. We extracted the Cry proteins, ran the SDS PAGE (two Cry protein bands were observed), and tried to identify the composition of the two Cry protein bands in the SDS PAGE. The bioinformatics pipeline is described as follows: First, we converted the original mass spectrum files to the mgf file (peaks file), then the mgf files were searched against the Bacillusthuringiensis CT-43 protein database using Mascot (version 2.3.02). The search parameters were: i) trypsin was chosen as the enzyme with one missed cleavage allowed; ii) the fixed modifications of carbamidomethylation were set as Cys, and variable modifications of oxidation as Met; iii) peptide tolerance was set as 0.05 Da, and MS/MS tolerance was set as 0.1 Da. The peptide charge was set as Mr, and monoisotopic mass was chosen. An automatic decoy database search strategy was employed to estimate the false discovery rate (FDR). The FDR was calculated as the false positive matches divided by the total matches. In the final search results, the FDR was less than 1.5%.

2020-01-24 | PXD000425 | Pride

Bacillus thuringiensis F14-1

Project description:This strain will be used for comparative genome analysis

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Genome Sequences of Bacillus thuringiensis Serovar kurstaki Strain BP865 and B. thuringiensis Serovar aizawai Strain HD-133.

Project description: Not available

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Complete genome sequence of Bacillus thuringiensis serovar Sichuansis strain MC28.

Project description: Not available

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Bacillus thuringiensis stains induced systemic resistance in Brassica napus L.

Project description:Bacillus thuringiensis has insecticidal activity against a variety of important agricultural pests and exhibits good bacteriostatic resistance to a variety of plant pathogens, and recentily study have shown that two strains of Bt (B88-82 and RG1-6 Strain) can induce the tomato to produce resistance to R. solanacearum. However, only the induced signal pathway has been studied, and its active substances are not reported. The aim of this study was to further explore the Bt strain that could induce plant disease resistance and study the induced activity of the Bt strain, and to study the signal pathway induced by transcriptional sequencing and fluorescence quantitative PCR. The results showed that there were 303 differentially expressed genes in rape after induction of 4F5 strain, among which 86 genes were up-regulated and 217 genes weredown-regulated. The result of 4BM1 strain induction was induced by transcriptase sequencing. There were 126 differentially expressed genes in rape. Among which 64 genes were up-regulated and 62 genes were down-regulated. The analysis of these differentialexpression genes revealed that they contained Salicylic acid pathway and Ethylene pathway-related genes, which need to be further verified.

2018-08-03 | GSE118042 | GEO

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