Project description:Echinoderms, possessing outstanding regenerative capability, provide unique model system for the study of the response to injury. However, there is little known about the proteomic composition of coelomic fluid, an important biofluid circulating through the whole body and reflecting an overall biological status of the organism. In this study, we used LC-MALDI tandem mass spectrometry to characterize proteome of cell-free coelomic fluid of starfish Asterias rubens and follow the changes occurring in response to puncture wound and blood loss. Our study demonstrated significant changes of CF proteome during the first hours after injury and presented a series of candidate proteins involved in early response to injury, providing interesting targets for future functional studies.
Project description:Marine organisms represent a rich source for discovering natural products and materials that could inspire the development of novel molecules or materials for a high variety of industrial applications. Among them are sea stars, emblematic animals of the seashore. These organisms rely on epidermal secretions to cope with their benthic life. Their integument produces a mucus, which represents the first barrier against invaders; and their tube feet produce adhesive secretions to pry open mussels and attach strongly but temporarily to rocks. In this study, we investigate for the first time the protein content of mucous and adhesive secretions from the sea star Asterias rubens. These secretomes were analysed using tandem mass spectrometry and resulting MS/MS data were searched against in silico translated tube foot transcriptome. Tube foot transcripts coding for proteins identified in the two secretions were then functionally annotated by similarity searches against NCBI nr database.