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Proteome profiling of cell-free coelomic fluid from the common starfish Asterias rubens

ABSTRACT: Echinoderms, possessing outstanding regenerative capability, provide unique model system for the study of the response to injury. However, there is little known about the proteomic composition of coelomic fluid, an important biofluid circulating through the whole body and reflecting an overall biological status of the organism. In this study, we used LC-MALDI tandem mass spectrometry to characterize proteome of cell-free coelomic fluid of starfish Asterias rubens and follow the changes occurring in response to puncture wound and blood loss. Our study demonstrated significant changes of CF proteome during the first hours after injury and presented a series of candidate proteins involved in early response to injury, providing interesting targets for future functional studies.

INSTRUMENT(S): TOF/TOF 5800

ORGANISM(S): Asterias Rubens

TISSUE(S): Coelomic Fluid

SUBMITTER: Sergey Shabelnikov

LAB HEAD: Sergey Shabelnikov

PROVIDER: PXD010228 | Pride | 2019-03-15

REPOSITORIES: Pride

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Injury affects coelomic fluid proteome of the common starfish, <i>Asterias rubens</i>.

Shabelnikov Sergey V SV Bobkov Danila E DE Sharlaimova Natalia S NS Petukhova Olga A OA

The Journal of experimental biology 20190321 Pt 6

Echinoderms, possessing outstanding regenerative capabilities, provide a unique model system for the study of response to injury. However, little is known about the proteomic composition of coelomic fluid, an important biofluid circulating throughout the animal's body and reflecting the overall biological status of the organism. In this study, we used LC-MALDI tandem mass spectrometry to characterize the proteome of the cell-free coelomic fluid of the starfish <i>Asterias rubens</i> and to follo ...[more]

PMID: 30877231

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