Project description:Mycoplasma gallisepticum transcriptome comparison between in vitro grown cultures of strains Rlow and F utilizing oligo DNA microarrays.

2010-01-07 | GSE19755 | GEO

Transcription profiling of different state of Mycoplasma gallisepticum

Project description:Mycoplasma gallisepticum is a convenient model object for studying the regulation of transcription because it has a reduced genome, lack of cell wall and many metabolic pathways, and also easy to culture and non-pathogenic to humans. For rapid investigation of gene expression we developed microarray design including 3 366 probes for 678 genes. They included 665 protein coding sequences and 13 antisense RNAs from 816 genes and 17 ncRNAs present in Mycoplasma gallisepticum. This work was carried out transcriptomic profiling for different types of effects on the expression of genes of Mycoplasma gallisepticum: 1) genetic knock-out mutants; 2) cell culture exposed to sublethal concentrations of antibiotics; and 3) well-characterized heat stress effect. The study was performed on Agilent one-color microarray with custom design and random-T7 polymerase primer for cDNA synthesis. Using set of different probes for each gene or ncRNA allows to increase accuracy of gene expression quality.

2016-08-18 | GSE85777 | GEO

Mycoplasma gallisepticum Rlow attached to MRC-5 cells microarray

Project description:The goal of this study was to identify genes important to the interaction of Mycoplasma gallisepticum with host cells in an in vitro system. An interaction time of one hour was chosen since it is less than a generation time and thus all changes can be attributed to transcriptional changes. A total of 60 transcripts were determined to be significantly up- or down-regulated under these conditions, including several hypothetical genes. Also several metabolism-related genes and ATP synthase genes were significantly down-regulated. Keywords: transcriptional response to host cells

2007-05-20 | GSE6717 | GEO

Mycoplasma gallisepticum: Rlow vs. F

Project description:Mycoplasma gallisepticum transcriptome comparison between in vitro grown cultures of strains Rlow and F utilizing oligo DNA microarrays. Two-condition experiment, Rlow vs. F strain cells. Biological replicates: 3. 1 technical replicate per biological replicate which includes a dye swap.

2010-04-22 | E-GEOD-19755 | biostudies-arrayexpress

Data on nucleoid-associated proteins isolated from Mycoplasma gallisepticum after intracellular infection

Project description:Mycoplasma gallisepticum belongs to the class Mollicutes. It causes chronic respiratory disease in avian species. M. gallisepticum is characterized by lack of cell wall; reduced genome size and the volume of its nucleoid is comparable to the size of the whole cell. As a result of genome reduction, M. gallisepticum has a limited variety of DNA-binding proteins (DBP) and transcription factors. It was shown, however, that mycoplasmas demonstrate a wide range of differential expression in response to various stress factors, which promotes effective adaptation to unfavorable conditions. We assume that in the case of mycoplasmas, which are characterized by a combination of the reduction of known gene expression regulation systems and a high adaptive potential, the coordination of gene expression can be provided due to local changes in the structure and spatial organization of the chromosome. The study of the dynamic changes of the proteomic profile of M. gallisepticum nucleoid may assist in revealing its mechanisms of functioning, regulation of chromosome organization and stress adaptation including its changes upon invasion of the host cells.

2021-03-31 | PXD025107 | Pride

Proteomic response and variability of surface Vlh-antigens of bacteria Mycoplasma gallisepticum in the eukaryotic invasion model

Project description:Proteomic response and variability of surface Vlh-antigens of bacteria Mycoplasma gallisepticum in the eukaryotic invasion model

2016-04-08 | MSV000079642 | MassIVE

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