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Homo sapiens

ABSTRACT: A microfluidics-based in vitro model of the gastrointestinal human-microbe interface

OTHER RELATED OMICS DATASETS IN: MTBLS328

PROVIDER: PRJNA315665 | ENA |

REPOSITORIES: ENA

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A microfluidics-based in vitro model of the gastrointestinal human-microbe interface

Project description:Changes in the human gastrointestinal microbiome are associated with several diseases. To infer causality, experiments in representative models are essential, but widely used animal models exhibit limitations. Here we present a modular, microfluidics-based model (HuMiX, human–microbial crosstalk), which allows co-culture of human and microbial cells under conditions representative of the gastrointestinal human–microbe interface. We demonstrate the ability of HuMiX to recapitulate in vivo transcriptional, metabolic and immunological responses in human intestinal epithelial cells following their co-culture with the commensal Lactobacillus rhamnosus GG (LGG) grown under anaerobic conditions. In addition, we show that the co-culture of human epithelial cells with the obligate anaerobe Bacteroides caccae and LGG results in a transcriptional response, which is distinct from that of a co-culture solely comprising LGG. HuMiX facilitates investigations of host–microbe molecular interactions and provides insights into a range of fundamental research questions linking the gastrointestinal microbiome to human health and disease.

2017-07-28 | MTBLS328 | MetaboLights

A microfluidics-based in vitro model of the gastrointestinal human-microbe interface

Project description:We have developed a microfluidics-based in vitro model of the human gut allowing co-culture of human and microbial cells and subsequent multi-omic assessment of the effect of the co-culture on the host transcriptome. We compare the transcriptional changes induced in the human epithelial cell line, Caco-2 after co-culture with Lactobacillus rhamnosus GG or a consortium of Lactobacillus rhamnosus GG and Bacteroides caccae.

2016-05-05 | GSE79383 | GEO

LC-MS/MS analysis on in-vivo mice gastrointestinal digests

Project description:Food protein amyloid fibrils have superior technological,nutritional,sensorial,and physical properties compared to native monomers, butthere is yet insufficient understanding of their digestive fate and safety for wide consumption.By combining SDS,ELISA,fluorenscence,AFM, MALDI-MS, CD microfluidics,and SAXS techniques for the characterization of blg and lysozyme amyloid fibrils subjected to in-vitro gastrointestinal digestion.LC-MS/MS analysis on the fate of blg/lysozyme amyloid fibrils digested in vivo by mice was applied to verify our in vitro results.

2023-10-30 | PXD045698 | Pride

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Project description:Cytokines dominate the secretome of primary human myotubes in an in vitro exercise model

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Sven ES, SDS-PAGE

Project description:MS based secretome analyses of Strongyloides venezuelensis, a gastrointestinal parasite of rats that is widely used as a laboratory model and is known to produce both soluble and insoluble (adhesive) secretions during its parasitic stages.

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