Project description:Hfq proteins are RNA chaperones that play a critical role in post-transcription regulation of gene expression. Bacteria of the Burkholderia cepacia complex harbor two distinct and functional Hfq proteins, the Hfq and Hfq2. We have previously performed the functional analysis of Hfq and Hfq2 in the pathogen Burkholderia cenocepacia J2315. In order to examine the impacts of each RNA chaperone on the global transcriptome of B. cenocepacia J2315, we performed comparative transcriptome profile of mutants on the hfq and hfq2 genes, using as reference the wild-type strain.
Project description:[1] Transcription profiling of one Burkholderia cenocepacia clinical isolate, J2315, versus a soil isolate, HI2424, in conditions mimicking CF sputum [2] Transcription profiling of Burkholderia cenocepacia isolates J2315 and HI2424 in media mimicking CF sputum or the soil environment
Project description:Hfq proteins are RNA chaperones that play a critical role in post-transcription regulation of gene expression. Bacteria of the Burkholderia cepacia complex harbor two distinct and functional Hfq proteins, the Hfq and Hfq2. We have previously performed the functional analysis of Hfq and Hfq2 in the pathogen Burkholderia cenocepacia J2315. In order to examine the impacts of each RNA chaperone on the global transcriptome of B. cenocepacia J2315, we performed comparative transcriptome profile of mutants on the hfq and hfq2 genes, using as reference the wild-type strain. For expression profiling, over-night cultures of the Burkholderia cenocepacia J2315 wild-type strain and the isogenic mutants hfq::Tp and M-NM-^Thfq2 grown in LB medium were diluted to an initial OD640 nm of 0.25 into LB medium. Triplicate samples were cultured at 37M-BM-:C with 250 r.p.m. agitation for 16 h and RNA extracted from the three bacterial isolates.
Project description:The small RNA ncS35 was deleted from the Burkholderia cenocepacia J2315 genome. Gene expression of mutant was compared to wild type in three growth conditions: exponential phase and stationary phase planktonic growth and biofilm growth, all in LB medium.
Project description:Burkholderia cenocepacia J2315 was grown in LB broth with and without the presence of the desinfectant chlorhexidine in the subinhibitory concentration of 5mg/L. Four biological replicates for each control and test conditions were performed. The experiment was designed as a direct comparison with dye swap of two replicates.
Project description:[1] Transcription profiling of one Burkholderia cenocepacia clinical isolate, J2315, versus a soil isolate, HI2424, in conditions mimicking CF sputum [2] Transcription profiling of Burkholderia cenocepacia isolates J2315 and HI2424 in media mimicking CF sputum or the soil environment [1] J2315 vs. HI2424 cells in the same condition. [2] Two-condition experiment. Biological replicates: 4 replicates.
Project description:Burkholderia cenocepacia J2315 wild type and adaptive mutants with elevated resistance to antibiotics were exposed to sub-inhibitory concentrations of drugs
Project description:Burkholderia cenocepacia J2315 was grown in LB broth to the beginning of stationary phase. The expression profile was compared to cultures harvested in mid-log phase.
Project description:In order to unravel the molecular mechanisms involved in the resistance of biofilm-grown <br>Burkholderia cenocepacia cells against high concentrations of disinfectants, the present <br>study focussed on the transcriptional response in sessile B. cenocepacia J2315 cells<br> following exposure to high levels of H2O2, NaOCl or chlorhexidine. In addition differences<br> in gene expression were examined between untreated B. cenocepacia sessile cells and <br>B. cenocepacia planktonic cells.
