Project description:The vancomycin resistant strain V583 was exposed to therapeutical dose of vancomycin in order to understand the cell response to the antibiotic besides the induction of the vanB genes E. faecalis V583 cells were collected at 10 minutes and 30 min after vancomycin addition and without vancomycin, for RNA extraction and hybridization on Affymetrix microarrays. The cells were collected when at 0.4. For each condition were made replicates.

2014-01-01 | E-GEOD-52263 | biostudies-arrayexpress

Enterococcus faecium E1071

Project description:Vancomycin-resistant strain

| PRJNA29887 | ENA

Transcriptomics analysis of E. faecalis V583 exposed to subinhibitory concentration of chlorhexidine

Project description:The vancomycin resistant strain V583 was exposed to subinhibitory concentration of chlorhexidine. E. faecalis V583 cells were collected at 15 minutes after chlorhexidine addition and without chlorhexidine, for RNA extraction and hybridization on Affymetrix microarrays.

2017-01-11 | E-MTAB-5181 | biostudies-arrayexpress

Enterococcus faecalis

Project description:Complete genome sequences of linezolid-resistant and vancomycin-susceptible Enterococcus faecalis clinical isolates

| PRJDB6823 | ENA

Enterococcus faecalis OG1RF control vs. treatment with vancomycin

Project description:Transcriptional profiling to investigate the effect of drug treatment on the E. faecalis cells. For microarray analysis, E. faecalis OG1RF was grown in FMC medium supplemented with 10 mM glucose to an optical density at 600 nm (OD600) of 0.3 and the cultures were divided in 3 aliquots. One aliquot was collected by centrifugation and immediately frozen (untreated control cells). The other aliquots were treated for 30 or 60 min with 1.25 X the minimum inhibitory concentration (MIC) of vancomycin (10 M-NM-<g ml-1). After an exposure time of 30 or 60 minutes, each of these cultures was also centrifuged and the pellets frozen. RNA was then isolated from each pellet for microarray analysis. This process was repeated 3 additional times, for a total of four replicates of each condition. RNA was extracted from four replicate samples of each condition of interest (control cells grown to OD600 = 0.3 in FMC medium supplemented with 10mM glucose, then treated with vancomycin for 30 or 60 minutes) and labeled with Cy3. For each replicate, labeled RNA was hybridized to slides along with Cy5-labeled reference RNA, extracted from E. faecalis OG1RF cells grown in BHI medium to mid-log.

2013-03-19 | E-GEOD-45300 | biostudies-arrayexpress

Gene expression profiles of differentially expressed genes in Enterococcus faecalis 14 ΔpnpA mutant strain

Project description:To explain the particular behavior of the mutant ΔpnpA strain, we performed a comparative transciptomic analysis of RNA isolated from a derivative strain ΔpnpA mutant versus the wild-type (WT) , after 3h and 6 h of growth in GM17 medium under semi-aerobic conditions.

2023-04-20 | GSE180397 | GEO

Enterococcus faecalis OG1RF control vs. treatment with vancomycin

2013-03-19 | GSE45300 | GEO

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