Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transient RNA-DNA Hybrids Are Required for Efficient Double-Strand Break Repair

ABSTRACT: Transient RNA-DNA Hybrids Are Required for Efficient Double-Strand Break Repair

PROVIDER: PRJNA335431 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Transient RNA-DNA Hybrids Are Required for Efficient Double-Strand Break Repair

Project description:RNA-DNA hybrids are a major internal cause of DNA damage within cells, and their degradation by RNAse H enzymes is important for maintaining genomic stability. Here, we identified an unexpected role for RNA-DNA hybrids and RNase H enzymes in DNA repair. Using a site-specific DNA double-stranded break (DSB) system in Schizosaccharomyces pombe, we showed that RNA-DNA hybrids form as part of the homologous recombination (HR)-mediated DSB repair process and RNase H enzymes are essential for their degradation and efficient completion of DNA repair. Deleting RNase H stabilizes RNA-DNA hybrids around DSB sites and strongly impairs recruitment of the ssDNA-binding RPA complex. In contrast, overexpressing RNase H1 destabilizes these hybrids, leading to excessive strand resection and RPA recruitment, and to severe loss of repeat regions around DSBs. Our study challenges the existing model of HR-mediated DSB repair, and reveals a surprising role for RNA-DNA hybrids in maintaining genomic stability.

2016-10-28 | GSE84882 | GEO

Transient RNA-DNA Hybrids Are Required for Efficient Double-Strand Break Repair

2016-10-28 | GSE80398 | GEO

Transient RNA-DNA Hybrids Are Required for Efficient Double-Strand Break Repair

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-10-28 | GSE84883 | GEO

Schizosaccharomyces pombe

Project description:Transient RNA-DNA Hybrids Are Required for Efficient Double-Strand Break Repair

| PRJNA318794 | ENA

Schizosaccharomyces pombe

Project description:Transient RNA-DNA Hybrids Are Required for Efficient Double-Strand Break Repair

| PRJNA335432 | ENA

p53 binding during the DNA double-strand break response

Project description:We used ChIP-seq to assess where p53 binds in the human genome and how that binding changes during the DNA double-strand break response. In particular, we considered the 1-Mb-wide window centered on the MYC locus. Contrary to previous reports, we found no evidence of p53 binding at the MYC promoter. Rather, we identified three locations downstream of MYC at which p53 was bound; binding at each of these regions increased during the DNA double-strand break response.

2017-08-02 | GSE101737 | GEO

CGH of Drosophila Follicle Cell Amplicons in double-strand break repair mutants

Project description:CGH of stage 13 amplifying follicle cells to measure changes in replication fork progression in double-strand break repair mutants Comparative genomic hybridization was performed to compare amplification gradients of stage 13 follicle cells from several double-strand break repair mutants to wild type (OrR) gradients. Two-three replicates were done for each genotype.

2017-02-08 | GSE86012 | GEO

RecG Directs DNA Synthesis During Double-Strand Break Repair [ChIP-seq]

Project description:In the bacterium Escherichia coli, RecG directs DNA synthesis during the repair of DNA double-strand breaks by homologous recombination. Examination of RecA binding during double-strand break repair in Escherichia coli in the presence and absence of RecG protein

2016-05-19 | E-GEOD-77181 | biostudies-arrayexpress

Distribution of Holliday Junctions and Repair Forks during Escherichia coli DNA Double-Strand Break Repair

Project description:Here, we correlated and compared two different steps of the Double-Strand Break Repair pathway: RecA loading and Holliday junction formation

2021-07-29 | GSE179963 | GEO

DNA Double Strand Break Repair in E. coli Perturbs Cell Division and Chromosome Dynamics

Project description:The effect of a site-specific DNA double-strand break on the abundance of E. coli chromosomal DNA during exponential growth was investigated by marker frequency analysis (MFA).

2020-01-20 | GSE141011 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data