Genomics

Dataset Information

23

Transient RNA-DNA Hybrids Are Required for Efficient Double-Strand Break Repair


ABSTRACT: RNA-DNA hybrids are a major internal cause of DNA damage within cells, and their degradation by RNAse H enzymes is important for maintaining genomic stability. Here, we identified an unexpected role for RNA-DNA hybrids and RNase H enzymes in DNA repair. Using a site-specific DNA double-stranded break (DSB) system in Schizosaccharomyces pombe, we showed that RNA-DNA hybrids form as part of the homologous recombination (HR)-mediated DSB repair process and RNase H enzymes are essential for their degradation and efficient completion of DNA repair. Deleting RNase H stabilizes RNA-DNA hybrids around DSB sites and strongly impairs recruitment of the ssDNA-binding RPA complex. In contrast, overexpressing RNase H1 destabilizes these hybrids, leading to excessive strand resection and RPA recruitment, and to severe loss of repeat regions around DSBs. Our study challenges the existing model of HR-mediated DSB repair, and reveals a surprising role for RNA-DNA hybrids in maintaining genomic stability. Overall design: Inducible Double Strand Break (DSB) system. 6 samples, all in duplicates (12 samples alltogether) Insert sequence file (DSB.system.insert.txt); modified S. pombe genome sequence (Schizosaccharomyces_pombe.ASM294v2.29.dna.genome.modified_DSBsystem03032016.fa), and modified S. pombe annotation file (Schizosaccharomyces_pombe.ASM294v2.29.dna.genome.modified_DSBsystem03032016.bed)

INSTRUMENT(S): Illumina HiSeq 2000 (Schizosaccharomyces pombe)

SUBMITTER: Tamas Fischer  

PROVIDER: GSE80398 | GEO | 2016-10-28

SECONDARY ACCESSION(S): PRJNA318794

REPOSITORIES: GEO

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Publications

Transient RNA-DNA Hybrids Are Required for Efficient Double-Strand Break Repair.

Ohle Corina C   Tesorero Rafael R   Schermann Géza G   Dobrev Nikolay N   Sinning Irmgard I   Fischer Tamás T  

Cell 20161027 4


RNA-DNA hybrids are a major internal cause of DNA damage within cells, and their degradation by RNase H enzymes is important for maintaining genomic stability. Here, we identified an unexpected role for RNA-DNA hybrids and RNase H enzymes in DNA repair. Using a site-specific DNA double-strand break (DSB) system in Schizosaccharomyces pombe, we showed that RNA-DNA hybrids form as part of the homologous-recombination (HR)-mediated DSB repair process and that RNase H enzymes are essential for their  ...[more]

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