Project description:The seed coat is a vital tissue for directing nutrient supply to the embryo and cotyledons during development. By forming a sucrose gradient, the seed coat promotes transport of sugars from source leaves to seeds, thereby increasing sink strength. Understanding how gene regulation in sink tissue is altered by climate change factors will help elucidate the role these genes play in determining yield. This project aims to determine how elevated temperature, drought and ozone alter gene expression in the seed coat. Overall this study discovered high abundance seed coat specific genes, which may be candidates for functional genomic analysis in the future.
Project description:Glycine max was cultivated in China for nearly 5000 years, commonly referred to as soybeans, now it has become one of the important economic crops in the world (Li et al., 2008). Post-translational modifications are known to regulate many cellular processes, which are dynamic and reversible and can make protein functions changed.(Westermann and Weber, 2003). To date, among 400 PTMs have been detected, such as Acetylation, Ubiquitination, Phosphorylation, Malonylation, Succinylation and Methylation(Colak et al., 2013; Weinert et al., 2013).
Project description:A transcriptome analysis of soybean seeds harvested at different developing stages (between stage 7.1 and stage 9) was carried out to understand the molecular events occuring during the acquisition of seed longevity during maturation.
Project description:Cytosine methylation is a base modification that is often used by genomes to store information that is stably inherited through mitotic cell divisions. Most cytosine DNA methylation is stable throughout different cell types or by exposure to different environmental conditions in plant genomes. Here, we profile the epigenomes of ~100 Glycine max lines to explore the extent of natural epigenomic variation. We also use these data to determine the extent to which DNA methylation variants are linked to genetic variations.
