Auxin production in the endosperm drives seed coat development in Arabidopsis
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA339548 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description: Not available
| S-EPMC5135394 | biostudies-literature
Project description:The endosperm is an ephemeral tissue that nourishes the developing embryo, similar to the placenta in mammals. In most angiosperms, endosperm development starts as a syncytium, in which nuclear divisions are not followed by cytokinesis. The timing of endosperm cellularization largely varies between species, and the event triggering this transition remains unknown. Here we show that increased auxin biosynthesis in the endosperm prevents its cellularization, leading to seed arrest. Auxin-overproducing seeds phenocopy paternal-excess triploid seeds derived from hybridizations of diploid maternal plants with tetraploid fathers. Concurrently, auxin-related genes are strongly overexpressed in triploid seeds, correlating with increased auxin activity. Reducing auxin biosynthesis and signaling reestablishes endosperm cellularization in triploid seeds and restores their viability, highlighting a causal role of increased auxin in preventing endosperm cellularization. We propose that auxin determines the time of endosperm cellularization, and thereby uncovered a central role of auxin in establishing hybridization barriers in plants.
2019-12-21 | GSE124108 | GEO
Project description:In angiosperms, the mature seed consists of an embryo (E), a seed coat (SC), and, in many cases, an endosperm. In contrast to knowledge about embryo and endosperm, we have relatively little knowledge of SC, especially at the genomics level. In this study, we analyzed the gene expression during seed development using the panel of cultivated and wild pea genotypes. We report the comprehensive gene expression changes related both to development as well as domestication status. Analysis of seed developmental stages revealed extensive modification of gene expression between wild pea progenitor and cultivated pea crop. A significant difference in gene expression dynamics appeared between early and late developmental stages D1, D2, and D3, D4, D5 in wild pea genotypes, where the expression was increased 3-5-fold and 5-10-fold, respectively. Our work extends knowledge about the role of the seed coat during pea seed development. We described gene expression dynamic resulting in specific metabolic profiles providing new insight into pea domestication.
2024-03-18 | GSE244961 | GEO
Project description:This SuperSeries is composed of the SubSeries listed below.
2016-11-15 | GSE85848 | GEO
Project description:Tissues were isolated from the globular seed using laser microdissection. At least two bioreplicates are included for each tissue. Tissues examined are : the embryo proper (EP), micropylar endosperm (MCE), peripheral endosperm (PEN), chalazal endosperm (CZE), chalazal proliferating tissue (CPT), chalazal seed coat (CZSC), inner seed coat (ISC), and outer seed coat (OSC).
2019-01-15 | GSE120360 | GEO
Project description:Transcriptional profiling during Arabidopsis seed coat development at 3 key developmental timepoints by using 2 mutant lines and their wild types. The data provides a globe view of seed coat development in arabidopsis can be used for identification of new gene candidates for seed coat development.
2011-03-10 | GSE27857 | GEO
Project description:The endosperm is a reproductive tissue supporting embryo development. In most 30 flowering plants, the initial divisions of endosperm nuclei are not succeeded by 31 cellularization; this process occurs only after a specific number of mitotic cycles have 32 taken place. The timing of cellularization significantly influences seed viability and size. 33 Previous research implicated auxin as a key factor in initiating nuclear divisions and 34 determining the timing of cellularization. Here, we uncover the involvement of a family 35 of clustered auxin response factors (cARFs) as dosage-sensitive regulators of 36 endosperm cellularization. cARFs, maternally expressed and paternally silenced, are 37 shown to induce cellularization, thereby restricting seed growth. Our findings align with 38 the predictions of the parental conflict theory, suggesting that cARFs represent major 39 molecular targets in this conflict. We further demonstrate a recurring amplification of 40 cARFs in the Brassicaceae, suggesting an evolutionary response to parental conflict 41 by reinforcing maternal control over endosperm cellularization. Our study highlights 42 that antagonistic parental control on endosperm cellularization converges on auxin 43 biosynthesis and signaling.
2024-03-22 | GSE232803 | GEO
Project description:We collected heart stage seed compartments from 7 micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments from seeds containing heart stage embryos. For the purposes of this study we captured 6 compartments: embryo proper, micropylar endosperm, peripheral endosperm, chalazal endosperm, chalazal seed coat and seed coat, as well sets of serial sections encompassing the entire heart stage seed. Experiment Overall Design: Heart stage seed compartments were isolated using the LMD6000 system. Total RNA was amplified and hybridized with Affymetrix ATH1 Arabidopsis array for 16 samples (embryo proper, micropylar endosperm, peripheral endosperm, seed coat and whole seeds, 2 biological replicates each; chalazal endosperm, chalazal seed coat, 3 biological replicates each).
2009-03-21 | E-GEOD-15160 | biostudies-arrayexpress
Project description:We collected heart stage seed compartments from 7 micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments from seeds containing heart stage embryos. For the purposes of this study we captured 6 compartments: embryo proper, micropylar endosperm, peripheral endosperm, chalazal endosperm, chalazal seed coat and seed coat, as well sets of serial sections encompassing the entire heart stage seed.
2009-03-12 | GSE15160 | GEO
Project description:We collected mature green seed compartments from 7 micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments from seeds containing mature green-stage embryos. For the purposes of this study we captured 6 compartments: embryo proper, micropylar endosperm, cellularized peripherial endosperm, chalazal endosperm, chalazal seed coat and seed coat, as well sets of serial sections encompassing the entire mature green stage seed. Experiment Overall Design: Mature green stage seed compartments were isolated using the LMD6000 system. Total RNA was amplified and hybridized with Affymetrix ATH1 Arabidopsis array for 14 samples (embryo proper, micropylar endosperm, peripherial endosperm, chalazal endosperm, chalazal seed coat, seed coat and whole seeds, 2 biological replicates each).
2009-03-21 | E-GEOD-15165 | biostudies-arrayexpress