Project description:This data presents the complete proteomic subcellular characterization (extracellular secreted proteins, cell wall-associated proteins, cytosolic proteins, crude membrane proteins, peripheral membrane proteins and proteins present in purified membranes) of Streptococcus pyogenes serotype M1 (strain AP1).

2017-04-20 | MSV000080971 | MassIVE

Streptococcus pyogenes (GAS) membrane vesicles (MVs) and membranes LC-MSMS

Project description:Proteomics characterisation of membrane vesicles (MV) and corresponding membranes derived from Streptococcus pyogenes M1 (clinical isolate ISS3348) grown to late-logarithmic phase in THB media.

2016-10-18 | PXD005167 | Pride

Effects of RNase Y mutation on operon organization of Streptococcus pyogenes NZ131

Project description:Purpose: RNase Y is a major enzyme responsible for mRNA degradation in Streptococcus pyogenes. The goals of this study are to understand whether RNase Y plays a role in operon transcription of S. pyogenes NZ131 by using RNA-seq analysis. Methods: S. pyogenes mRNA profiles of wild type (WT) and RNase Y mutant (∆rny) were generated by deep sequencing, in duplicate, using Illumina Hiseq 2000. The sequence reads were aligned to the S. pyogenes genome using Bowtie2. The aligned files were sorted to BAM format and indexed using Samtools. The read depth of each base was derived from BAM files using BEDtools. Operon organization of S. pyogenes WT and ∆rny strains were predicted based on base reads. Results: A total of 11 to 12 billion reads were obtained from each sample. More than 99% of these reads were mapped to the S. pyogenes genome. Predictions of operon organization using WT and ∆rny samples showed little difference between the two strains. Conclusions: Our result shows that the mutation of RNase Y does not affect the operon organization of S. pyogenes NZ131.

2017-06-02 | GSE99533 | GEO

Streptococcus pyogenes M1 GAS isolate:SF370

Project description:Differential gene expression in Streptococcus pyogenes WT, Δrnc and Δrny

| PRJNA341673 | ENA

Multiple roles of RNase Y in Streptococcus pyogenes mRNA metabolism

Project description:Streptococcus pyogenes (Group A streptococcus, GAS) is an important human pathogen that causes a variety of infectious diseases and sequelae. Recent studies showed virulence factor expression was controlled at multiple levels, including the post-transcriptional regulation. In this study, we examined the global half-lives of S. pyogenes mRNAs and explored the role RNase Y played in mRNA metabolism with microarray analysis. key word: genetic modification Streptococcus pyogenes NZ131 wild-type cells and ?rny strains were grown in C-medium until late exponential phase. Rifampicin was added to the cell culture and samples were collected before and after rifampicin addition. The transcriptional profile of the whole genome before and after rifampicin addition was examined with microarray. Please note that mRNA decay assay resulted in considerable variations in the datasets. Samples were taken after rifampicin addition and subsequent incubation for different time intervals. During that time no new RNA is produced and the remaining RNA is degraded to various degrees.

2013-02-01 | E-GEOD-40198 | biostudies-arrayexpress

Gene expression of GidA mutant Streptococcus pyogenes

Project description:In Streptococcus pyogenes, mutation of GidA results in avirulence despite the same growth rate as the wild type. To understand the basis of this effect, global transcription profiling was conducted. Keywords: Wild type vs. GidA mutant Streptococcus pyogenes

2008-01-10 | GSE9678 | GEO

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