Dataset Information


Homo sapiens

ABSTRACT: Effect of serum extracellular vesicle depletion protocols on miRNA expression of cultured cells

ORGANISM(S): Homo sapiens  



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Serum extracellular vesicle depletion processes affect release and infectivity of HIV-1 in culture.

Liao Zhaohao Z   Muth Dillon C DC   Eitan Erez E   Travers Meghan M   Learman Lisa N LN   Lehrmann Elin E   Witwer Kenneth W KW  

Scientific reports 20170531 1

Extracellular vesicles (EVs) are involved in intercellular communication and affect processes including immune and antiviral responses. Blood serum, a common cell culture medium component, is replete with EVs and must be depleted prior to EV-related experiments. The extent to which depletion processes deplete non-EV particles is incompletely understood, but depleted serum is associated with reduced viability and growth in cell culture. Here, we examined whether serum depleted by two methods affe  ...[more]

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