Project description:Novosphingobium resinovorum strain SA1 is one of few strains capable of degrading sulfanilic acid which is a widely used representative of sulfonated aromatic compounds. In order to identify the elements involved in the biodegradation process and to understand the metabolic responces of the cells exposed to this aromatic compound, we performed a whole transcriptome analysis of cells grown on sulfanilic acid and glucose. Additionally, for distinguish the potential stress/starvation effects of the xenobiotic we compared the transcript profiles of samples taken from both the exponential and stationary growth phases.

2017-08-15 | GSE102626 | GEO

Metabolic capacities of Novosphingobium sp. HR1a in the rhizosphere

Project description:Rhizoremediation, the biotechnology of the utilization of rhizospheric microorganisms associated with plant roots for the elimination of soil contaminants, is based on the ability of microorganisms to metabolize nutrients from plant root exudates, in order to survive the stressful conditions of the rhizosphere, and thereby, to co-metabolize or even mineralize toxic environmental contaminants. Novosphingobium sp. HR1a is a bacterial strain able to degrade a wide variety of polycyclic aromatic hydrocarbons (PAHs). We have demonstrated that this bacterium is able to grow in vegetated microcosms and to eliminate phenanthrene in the presence of clover faster than in non-vegetated systems, establishing a positive interaction with clover. We have studied the molecular basis of this interaction by phenomic, metabolomic and transcriptomic analyses, demonstrating that the positive interaction between clover and Novosphingobium sp. HR1a is a result of the bacterial utilization of different carbon and nitrogen sources (such as sugars, amino acids and organic acids) released during seedling development, and the capacity of exudates to induce the PAH degradation pathway. These results are pointing out to Novosphingobium sp. HR1a as a promising strain for the bioremediation of PAH-contaminated soils.

2021-04-19 | GSE172273 | GEO

Novosphingobium barchaimii strain:NS277

Project description:Novosphingobium barchaimii Genome Sequencing

| PRJNA278216 | ENA

Aromatic dimer dehydrogenases from Novosphingobium aromaticivorans reduce aromatic diketones

Project description:Aromatic diketones are a major product of formic acid lignin depolymerization. Novosphingobium aromaticivorans can degrade these diketones, but the enzymes used in this process were unknown. We used RNA-Seq to identify aromatic dimer dehydrogenases as potential candidates for the initial reduction of the aromatic G-diketone, then verified this using in vitro enzyme assays.

2021-05-22 | GSE174697 | GEO

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