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Escherichia coli strain:BW25113 JME44

ABSTRACT: Escherichia coli BW25113 JME44 resequencing genome sequencing

PROVIDER: PRJNA370162 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8846859_1.fastq.gz	Fastqsanger.gz
	SRR8846859_2.fastq.gz	Fastqsanger.gz

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Complete Genome Sequence of Escherichia coli BW25113.

Project description:Escherichia coli BW25113 is the parent strain of the Keio collection comprising nearly 4,000 single-gene deletion mutants. We report the complete 4,631,469-bp genome sequence of this strain and the key variations from the type strain E. coli MG1655.

2014-01-01 | S-EPMC4200154 | BioStudies

Pseudogene product YqiG is important for pflB expression and biohydrogen production in Escherichia coli BW25113.

Project description:Pseudogenes in the Escherichia coli genome are assumed to be non-functional. In this study, Keio collection BW25113∆yqiG and YqiG-producing strain (BW25113/pCA24N-YqiG) were used to evaluate the importance of pseudogene yqiG in hydrogen metabolism. Our results show pseudogene protein YqiG was identified as an essential protein in the production of biohydrogen from glucose. The mutant yqiG decreased biohydrogen production from 37 µmol mg-1 protein to 6 µmol mg-1 protein compared to the wild-type strain, and glucose consumption was reduced by 80%. Through transcriptional analysis, we found that the yqiG mutation represses pflB transcription tenfold; pflB encodes pyruvate-formate lyase, one of the key enzymes in the anaerobic metabolism of E. coli. Moreover, production of YqiG stimulated glycolysis and increased biohydrogen productivity 1.5-fold compared to that of the wild-type strain. Thus, YqiG is important for the central glycolysis reaction and is able to influence hydrogen metabolism activity in E. coli.

2018-01-01 | S-EPMC6170319 | BioStudies

Microarray analysis of RNase I-deficient Escherichia coli versus wild-type BW25113

Project description:The present study investigated the role(s) of RNase I (encoded by the rna gene) in Escherichia coli by comparative gene expression analysis of an rna mutant and the isogenic wild-type E. coli strain BW25113. The transcriptomic analysis aims to provide mechanistic insight into aberrant phenotypes observed in the RNase I-deficient mutant.

2020-01-04 | E-MTAB-6095 | BioStudies

Transcriptional responses of Escherichia coli rpoS- BW25113 vs. wild-type BW25113 under 15% ethanol shock in log phase

Project description:RpoS, an alternative sigma factor, is critical for stress response in Escherichia coli.RpoS also acts as a global regulator for stress control of gene expression, and actually dose so in log stage and stationary stage. To further understand the effect of environmental stresses on in ethanologenic strains, DNA microarrys was used to analyze the expression profiles of E. coli and its rpoS mutant strain. Overall design: BW25113（rpoS-）and BW25113 were selected at log stages and stationary stage of early development for RNA extraction and hybridization on Affymetrix microarrays. To that end, we hand-selected BW25113 and BW25113（rpoS-） according at different treatments: BW25113（rpoS-）at log stage (BW25113(rpoS-) log), BW25113 at log stage (BW25113 log),BW25113（rpoS-）atstationary stage ( BW25113(rpoS-) stationary), BW25113 at stationary stage ( BW25113 stationary)

2010-07-20 | GSE17526 | GEO

Escherichia coli strain:BW25113 JME70

Project description:Escherichia coli BW25113 JME70 resequencing genome sequencing

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Escherichia coli strain:BW25113 JME61

Project description:Escherichia coli BW25113 JME61 resequencing genome sequencing

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Escherichia coli strain:BW25113 JME76

Project description:Escherichia coli BW25113 JME76 resequencing genome sequencing

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Escherichia coli strain:BW25113 JBx_072908

Project description:Escherichia coli BW25113 JBx_072908 resequencing genome sequencing

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Escherichia coli strain:BW25113 JME45

Project description:Escherichia coli BW25113 JME45 resequencing genome sequencing

| PRJNA370163 | ENA

Escherichia coli strain:BW25113 JME37

Project description:Escherichia coli BW25113 JME37 resequencing genome sequencing

| PRJNA370161 | ENA

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