Project description:We investigated how Tfh cells contribute to the breakdown of peripheral immune tolerance and the formation of autoimmune lesions in a murine model of SS. Our results indicate a yet unreported mechanism of autoreactive response through Tfh cells in a SS model mouse, and it will be supportive for understanding the pathogenesis of autoimmunity. Overall design: Two-condition experiment, purified CD4+ CD25- T cells from spleen of non-thymectomied NFS/sld (Control) vs. purified CD4+ CD25- T cells from spleen of thymectomied NFS/sld (SS model).
Project description:Many plant researchers have applied genomic tools to model species to identify abiotic stress responsive genes that might be useful for improving stress tolerance in crops. However, it is unclear whether this translational approach will be successful given the complexity of abiotic stress tolerance. We carried out a functional genomic (ionomic, transcriptomic and metabolomic) comparison of three model and three forage species of the genus Lotus with varying tolerance to salinity. Transcriptome analysis showed that about 60 % of expressed genes were responsive to salt treatment in one or more of the six species tested, but less than 1 % was responsive in all genotypes. Therefore, genotype-specific responses overshadowed conserved transcriptional responses to salinity and represent an impediment to translational genomics. Fortunately, 'triangulation' from multiple species enabled the identification of a core set of conserved and tolerant-specific responses that could provide durable tolerance across genotypes.
Project description:To assess the effect of sleep deprivation on glucose metabolism and elucidate the mechanism, we established the mouse model wth C57BL/6J that is useful for the intervention on sleep deprivation associated diabetes and evaluate the liver metabolism and gene expression. Single six hours sleep deprivation induced increased hepatic glucose production assessed by pyruvate tolerance test and the hepatic triglyceride content was significantly higher in the sleep deprivation group than freely sleeping control group. Liver metabolites such as ketone bodies were increased in sleep deprivation group. Some gene expressions which associated with lipogenesis were increased. Overall design: In order to disturb the sleep, the cage changing method, the water platform method and the gentle handling method was performed using the C57BL/6J male mice. Intraperitoreal glucose tolerance tests were performed with each model. Using the model which shown impaired glucose tolerance by sleep deprivation, the plasma glucose and insulin levels were evaluated. Triglyceride content assay, metabolomics analysis, gene expression analysis by real-time PCR and gene microarray were also performed with liver tissue.
Project description:The wheat Nils expression profiles differ because of their response to aphid feeding (i.e., antibiosis - Dn1; tolerance - Dn2; antixenosis and antibiosis - Dn5) We used GeneChip Wheat Genome Array (Affymetrix, USA) analysis to detail the global programme of gene expression underlying cellularisation and identified distinct classes of up-regulated genes during this process. Overall design: Plants of each cultivar were infested with 10 aphids and incubated for 48 h. All leaves except the first leaf were harvested into liquid N2 and stored at 280°C prior to RNA isolation. Data analysis was conducted using CranBioconductor and using a linear mixed statistical model (Botha et al. 2014, Biology Open (2014) 3, 1116–1126 doi:10.1242/bio.201410280).