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Open chromatin in somatic cells transplanted into Xenopus oocytes

ABSTRACT: Open chromatin in somatic cells transplanted into Xenopus oocytes

PROVIDER: PRJNA386179 | ENA |

REPOSITORIES: ENA

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Open chromatin in somatic cells transplanted into Xenopus oocytes

Project description:Active gene transcription is associated with an open chromatin configuration at gene regulatory regions. However, the relationship between chromatin architecture of somatic cells and transcriptional activation during nuclear reprogramming in oocytes is unclear. We therefore examined changes in open chromatin states before and after transplantation of mouse somatic nuclei into Xenopus laevis oocytes by using the Assay for Transposase-Accessible Chromatin Sequencing (ATAC-seq).

2018-07-10 | GSE98776 | GEO

Transcriptional profiling of somatic cells differentiation throughout oocyte competence acquisition in the xenopus ovarian follicles.

Project description:Somatic cells surrounding the oocyte were sampled at the following stages: developmentally incompetent or poorly competent prophase I oocytes (NC1 oocytes), developmentally competent prophase I oocytes (C1 oocytes), and developmentally competent metaphase II oocytes (C2 oocytes). NC1 samples were collected from immature stage IV follicles, C1 samples from immature stage VI follicles, and C2 samples from in vitro matured stage VI follicles. Global transcriptional profiling was performed using somatic cells collected from xenopus ovarian follicles during in vivo oocyte developmental competence acquisition. Somatic cells were collected at 3 stages of oogenesis: early stage follicles (stage IV, vitellogenic, prophase I arrested oocytes, meiotically competent but developmentally incompetent, n=5), late stage follicles (stage VI, post-vitellogenic, prophase I arrested oocytes, meiotically competent and developmentally competent, n=5) and ovulatory follicles collected after in vitro maturation induction with hCG of post-vitellogenic follicles (metaphase II arrested oocytes, developmentally fully competent, n=5).

2012-10-14 | E-GEOD-36603 | biostudies-arrayexpress

Xenopus laevis oocyte mitochondria

Project description:Characterization of mitochondria in non-growing oocytes from xenopus laevis compared to somatic controls of the following tissues: heart, liver and white adipose tissue.

2022-05-09 | PXD030576 | Pride

Next generation sequencing identifies differentially localized transcripts in Xenopus laevis and Xenopus tropicalis oocytes

2015-12-09 | GSE58420 | GEO

Next generation sequencing identifies differentially localized transcripts in Xenopus laevis and Xenopus tropicalis oocytes

Project description:RNA-seq technology was used to identify differentially localized transcripts from Xenopus laevis and Xenopus tropicalis stage VI oocytes. Besides the discovery of a group of novel animally enriched RNAs, this study revealed a surprisingly low conservation of vegetal RNA localization between the two frog species. mRNA profiles of Xenopus laevis and Xenopus tropicalis animal and vegetal oocyte halves were generated by RNA-seq technology. For Xenopus laevis, animal and vegetal oocyte RNA preparations from two different females were generated in duplicates. For Xenopus tropicalis, animal and vegetal oocyte RNA preparations from two different females were analyzed.

2015-12-09 | E-GEOD-58420 | biostudies-arrayexpress

Xenopus oocytes reactivate muscle gene transcription in transplanted somatic nuclei independently of myogenic factors.

Project description: Not available

| S-EPMC2730400 | biostudies-literature

Microarray analysis of mRNAs enriched in the vegetal cortex of Xenopus oocytes

Project description:RNA localization is a fundamental mechanism for controlling the spatial regulation of protein synthesis within cells, as well as differential cell fates during early development. Localized RNAs are known to control critical aspects of early Xenopus development, but few have been studied in detail. We set out to identify novel transcripts localized to the vegetal cortex of Xenopus oocytes, one of the best-studied examples of RNA localization. We identified over 400 transcripts enriched in the vegetal cortex, compared with whole oocytes. Included were many novel genes, as well as known genes not thought to undergo RNA localization. These data suggest that the role of RNA localization in early development is extensive and will provide a resource for identifying candidate regulatory genes for early developmental processes. RNA was extracted from isolated vegetal cortices and compared with whole oocytes. Two biological replicates were used, each from a different donor female.

2009-12-30 | E-GEOD-17713 | biostudies-arrayexpress

Human oocytes reprogram somatic cells to a pluripotent state

Project description:This SuperSeries is composed of the following subset Series: GSE27507: Gene expression in pluripotent stem cells derived after somatic cell genome transfer into human oocytes GSE28022: Gene expression in blastomeres after transfer of somatic cells into human oocytes Refer to individual Series

2011-10-05 | E-GEOD-28024 | biostudies-arrayexpress

Microarray analysis of mRNAs enriched in the vegetal cortex of Xenopus oocytes

2009-12-31 | GSE17713 | GEO

Transcription profiling of mouse, bovine and xenopus germinal vesicle stage oocytes reveals a high degree of conservation among species

Project description:Comparative analysis of bovine, mouse and xenopus oocyte transcript profiles. The results from bovine and xenopus germinal vesicle stage oocytes in this experiment were compared to previously-published microarray data on preimplantation development in the mouse (Carter 2003).

2008-02-01 | E-MEXP-1339 | biostudies-arrayexpress

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