Project description:To characterize the transcriptional program that governs terminal granulocytic differentation in vivo, we performed comprehensive microarray analysis of human bone marrow population highly enriched for promyelocytes, myelocytes / metamyelocytes and neotrophils. Bone marrow and peripheral blood samples were collected from healthy individuals in parallel. Cell populations representing successive stages of terminal granulocytic differentation were isolated from human bone marrow samples by 2-layer density gradient centrifugation, neutrophils were collected from peripheral blood using 1-layer density gradient centrifugation. All populations were depleted of nongranulocytic cells by immunomagnetic sorting.
Project description:Normal human neutrophil development is a complex biological process, where the balance between cell proliferation, differentiation and apoptosis is tightly regulated by a transcriptional program that results in the production of appropriate numbers of circulating mature neutrophils. MicroRNAs (miRNAs) are small non-coding RNAs of 18~25 nt that affect cellular protein levels. Only limited data is available on miRNA expression patterns during normal granulocytic differentiation of primary human cells. We have examined miRNA expression patterns in distinct stages of granulocytic differentiation sorted from human bone marrow and identified highly stage-specific expressed miRNAs. Some of these differentially expressed miRNA are related to each other in terms of seed sequence (miRNA families) and location on the genome (miRNA clusters). These data reveal distinct expression patterns of specific miRNA sets that closely follow the transition of discrete maturation stages along the myeloblast-neutrophil pathway. In total 13 samples were measured, 2 myeloblast, 3 myeloblast/promyelocyte, 4 metamyelocytes and 3 neutrophils
Project description:Normal human neutrophil development is a complex biological process, where the balance between cell proliferation, differentiation and apoptosis is tightly regulated by a transcriptional program that results in the production of appropriate numbers of circulating mature neutrophils. MicroRNAs (miRNAs) are small non-coding RNAs of 18~25 nt that affect cellular protein levels. Only limited data is available on miRNA expression patterns during normal granulocytic differentiation of primary human cells. We have examined miRNA expression patterns in distinct stages of granulocytic differentiation sorted from human bone marrow and identified highly stage-specific expressed miRNAs. Some of these differentially expressed miRNA are related to each other in terms of seed sequence (miRNA families) and location on the genome (miRNA clusters). These data reveal distinct expression patterns of specific miRNA sets that closely follow the transition of discrete maturation stages along the myeloblast-neutrophil pathway.
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
Project description:Pathological processes like osteoporosis or steroid-induced osteonecrosis of the hip are accompanied by increased bone marrow adipogenesis. Such disorder of adipogenic/osteogenic differentiation, which affects also bone marrow derived mesenchymal stem cells (BMSCs) contributes to bone loss during aging. Therefore, we investigated the effects of extracellular vesicles (EVs) isolated from human (h)BMSCs during different stages of osteogenic differentiation on osteogenic and adipogenic differentiation capacity of naïve hBMSCs.
Project description:Transcriptional profiling of human mesenchymal stem cells comparing normoxic MSCs cells with hypoxic MSCs cells. Hypoxia may inhibit senescence of MSCs during expansion. Goal was to determine the effects of hypoxia on global MSCs gene expression.
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs. One-condition experment, gene expression of 3A6
Project description:Gene methylation profiling of immortalized human mesenchymal stem cells comparing HPV E6/E7-transfected MSCs cells with human telomerase reverse transcriptase (hTERT)- and HPV E6/E7-transfected MSCs. hTERT may increase gene methylation in MSCs. Goal was to determine the effects of different transfected genes on global gene methylation in MSCs.