Project description:To determine the global gene occupancy by Wiskott - Aldrich syndrome Protein (WASP) we perform ChIP-seq assay in two lymphoblastoid cell lines. We identify WASP-enriched genes, including several WASP-interaction genes previously reported; in addition, our results suggest the implication of WASP in diverse cellular process
2022-04-18 | GSE126483 | GEO
Project description:Outcomes of Hematopoietic Stem Cell Gene Therapy for Wiskott-Aldrich Syndrome, archive 1/2
| PRJNA781985 | ENA
Project description:Outcomes of Hematopoietic Stem Cell Gene Therapy for Wiskott-Aldrich Syndrome, archive 1 of 2
| PRJNA782743 | ENA
Project description:Outcomes of Hematopoietic Stem Cell Gene Therapy for Wiskott-Aldrich Syndrome, archive 2 of 2
Project description:This experiment intended to define differential gene expression between germinal center B cells expressing or not the Wiskott-Aldrich syndrome protein in mice. Sequencing was obtained on an Illumina HiSeq2500 system from Dark Zone GCB (DAPI-CD19+ GL7+IgD-CXCR4highCD86low) purified from CTL and GCBcWKO mice (n=4).
Project description:We knocked out Wiskott-Aldrich Syndrome protein (WASP) in an iPSC line and derived the cells to differentiate into macrophages. We found deficiency of WASP results in overexpression of splicing factors and irregulaly sized nulcear speckles. We performed DIA-MS based quantative proteome analysis for WASP wild-type and deficient macrophages.
Project description:The pleiotropic RTK Kit can provide cytoskeletal signals that define cell shape, positioning and migration, but the underlying mechanisms are less well understood. Here we provide evidence that Kit signals through WASP (Wiskott-Aldrich Syndrome Protein), the central hematopoietic actin nucleation- promoting factor and regulator of the cytoskeleton. KL-mediated gene expression in WT and WASP-deficient BMMCs was compared and revealed that approximately 30% of all Kit-induced changes were WASP-dependent. The results indicate that Kit signaling through WASP is necessary for normal Kit-mediated filopodia formation, cell survival and gene expression and provide new insight in the mechanism how WASP exerts a strong selective pressure in hematopoiesis.
Project description:Wiskott-Aldrich Syndrome-causative mutations disrupt alternative splicing and promote gene networks predisposed to hematologic malignancies
Project description:Wiskott-Aldrich Syndrome-causative mutations disrupt alternative splicing and promote gene networks predisposed to hematologic malignancies
