Transcription profiling of bone marrow derived mast cells from WASP negative and wild type mice to investigate the role of WASP in Kit signalling

ABSTRACT: The pleiotropic RTK Kit can provide cytoskeletal signals that define cell shape, positioning and migration, but the underlying mechanisms are less well understood. Here we provide evidence that Kit signals through WASP (Wiskott-Aldrich Syndrome Protein), the central hematopoietic actin nucleation- promoting factor and regulator of the cytoskeleton. KL-mediated gene expression in WT and WASP-deficient BMMCs was compared and revealed that approximately 30% of all Kit-induced changes were WASP-dependent. The results indicate that Kit signaling through WASP is necessary for normal Kit-mediated filopodia formation, cell survival and gene expression and provide new insight in the mechanism how WASP exerts a strong selective pressure in hematopoiesis.

ORGANISM(S): Mus musculus

SUBMITTER: Thomas Jahn 

PROVIDER: E-MEXP-2282 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress