Streptomyces tsukubensis NRRL18488
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ABSTRACT:
PROVIDER: PRJNA389523 | ENA |
REPOSITORIES: ENA
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Project description:In this work, we identified glucose and glycerol as tacrolimus repressing carbon sources in the important species Streptomyces tsukubaensis. A genome-wide analysis of the transcriptomic response to glucose and glycerol additions was performed using microarray technology. The transcriptional time series obtained allowed us to compare the transcriptomic profiling of S. tsukubaensis growing under tacrolimus producing and non-producing conditions. The analysis revealed important and different metabolic changes after the additions and a lack of transcriptional activation of the fkb cluster. In addition, we detected important differences in the transcriptional response to glucose between S. tsukubaensis and the model species Streptomyces coelicolor. A number of genes encoding key players of morphological and biochemical differentiation were strongly and permanently downregulated by the carbon sources. Finally, we identified several genes showing transcriptional profiles highly correlated to that of the tacrolimus biosynthetic pathway regulator FkbN that might be potential candidates for the improvement of tacrolimus production
2017-10-12 | GSE99752 | GEO
Project description:Chitin is the second most abundant biopolymer present in soils and is utilized by antibiotic-producing Streptomyces species. Its monomer, N-acetylglucosamine (NAG), regulates the developmental program of the model organism Streptomyces coelicolor. NAG blocks differentiation when growing on rich medium whilst it promotes development on poor culture media. We report here the negative effect of NAG on tacrolimus (FK506) production in Streptomyces tsukubaensis NRRL 18488 growing on a defined rich medium. Using microarrays technology, we found that GlcNAc represses the transcription of fkbN, encoding the main transcriptional activator of the tacrolimus biosynthetic cluster, and of ppt1, encoding a phosphopantheteinyltransferase involved in tacrolimus biosynthesis. On the contrary, NAG stimulated transcription of genes related to amino acid and nucleotide biosynthesis, DNA replication, RNA translation, glycolysis, pyruvate metabolism, and key gene members of the PHO regulon. The results obtained support those previously reported for S. coelicolor, but some important differences were observed
2020-02-08 | GSE110393 | GEO
Project description:Streptomyces tsukubaensis Genome sequencing and assembly
| PRJNA374465 | ENA
Project description:FK506 (tacrolimus) is a valuable immunosuppressant produced by several Streptomyces strains. In the genome of the wild type producer Streptomyces tsukubaensis NRRL18488 FK506 biosynthesis is encoded by a gene cluster that spans 83.5 kilobases. A whole transcriptome differential shotgun sequencing of S. tsukubaensis was performed to analyze transcription at two different time points; before and during active FK506 production. In total 8,914 transcription start sites were identified in either condition, which enabled precise determination of the 5'-UTR length of the corresponding transcripts as well as the identification of two consensus sequence motifs in the promoter regions. The transcription start sites of all gene operons within the FK506 cluster were identified, including three examples of leaderless RNA transcripts. These data provide detailed insight into the transcription of the FK506 biosynthetic gene cluster and supports future regulatory studies and genetic manipulations.
2017-07-05 | GSE92480 | GEO
Project description:The goal was to study the dfactionation of different lignocelullose (glucose, wheat bran, wheat straw) by Streptomyces coelicolor A3(2) and the corresponding production of secondary metabolites. This was performed by multi-omic experiment such as transcriptomic/metabolomic and leads to the production of new metabolites. For that, the strain Streptomyces coelicolor A3(2) was subjected to two carbon sources in triplicate (wheat bran and glucose as control). Enzymatic activities were studied at different times and the expression of CAZYmes was studied by transcriptomic in order to detect which enzymes are needed for each carbon source
2020-10-01 | E-MTAB-8971 | biostudies-arrayexpress
Project description:Streptomyces tsukubaensis NRRL 18488 is the preferred strain for the production of immunosuppressant agent tacrolimus (FK506). To take full advantage of its genetic potential, systematic understanding of secondary metabolism and related regulatory mechanisms is highly demanded. Here, to this end, we complete its 7.9 Mbp linear genome sequence followed by integrating with multi-omics measurements. With accurate reannotation of FK506 gene cluster, total 2,389 transcription start sites were determined by using primary transcriptome analysis. Integrated analysis of transcriptome and translatome data revealed that secondary metabolic gene clusters, especially FK506 cluster, undergo translational control with decrease in translational efficiency according to the growth. Furthermore, we demonstrated that SD motif has little correlation with ribosome pausing but AT-rich codons delay the translational elongation. Strong ribosome pausing was observed in the rare TTA codon in FK506 cluster. This comprehensive genome-scale analysis provides insight to the translational regulation of secondary metabolism in S. tsukubaensis.
2019-10-16 | GSE97637 | GEO
Project description:Ribosome pausing at the AT-rich codons regulates the protein expression of secondary metabolite gene clusters in the Streptomyces tsukubaensis NRRL 18488
| PRJNA382534 | ENA
Project description:RNA-seq Transcriptional Profiling of the FK506 Biosynthetic Gene Cluster in Streptomyces tsukubaensis NRRL18488 and General Analysis of the Transcriptome
| PRJNA357641 | ENA
Project description:Borodina2005 - Genome-scale metabolic network
of Streptomyces coelicolor (iIB711)
This model is described in the article:
Genome-scale analysis of
Streptomyces coelicolor A3(2) metabolism.
Borodina I, Krabben P, Nielsen
J.
Genome Res. 2005 Jun; 15(6):
820-829
Abstract:
Streptomyces are filamentous soil bacteria that produce more
than half of the known microbial antibiotics. We present the
first genome-scale metabolic model of a representative of this
group--Streptomyces coelicolor A3(2). The metabolism
reconstruction was based on annotated genes, physiological and
biochemical information. The stoichiometric model includes 819
biochemical conversions and 152 transport reactions, accounting
for a total of 971 reactions. Of the reactions in the network,
700 are unique, while the rest are iso-reactions. The network
comprises 500 metabolites. A total of 711 open reading frames
(ORFs) were included in the model, which corresponds to 13% of
the ORFs with assigned function in the S. coelicolor A3(2)
genome. In a comparative analysis with the Streptomyces
avermitilis genome, we showed that the metabolic genes are
highly conserved between these species and therefore the model
is suitable for use with other Streptomycetes. Flux balance
analysis was applied for studies of the reconstructed metabolic
network and to assess its metabolic capabilities for growth and
polyketides production. The model predictions of wild-type and
mutants' growth on different carbon and nitrogen sources agreed
with the experimental data in most cases. We estimated the
impact of each reaction knockout on the growth of the in silico
strain on 62 carbon sources and two nitrogen sources, thereby
identifying the "core" of the essential reactions. We also
illustrated how reconstruction of a metabolic network at the
genome level can be used to fill gaps in genome annotation.
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2015-07-30 | MODEL1507180049 | BioModels