Salmonella sp.
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ABSTRACT:
PROVIDER: PRJNA397772 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Salmonella Tn-seq under iron-restricted condition (Dip-100)
| PRJNA397770 | ENA
Project description:Salmonella Tn-seq under iron-restricted condition (Dip-250-II)
| PRJNA397774 | ENA
Project description:Salmonella Tn-seq under iron-restricted condition (Dip-250-I)
| PRJNA397773 | ENA
Project description:Salmonella Tn-seq under iron-restricted condition (Dip-400)
| PRJNA397775 | ENA
Project description:Salmonella Tn-seq under iron-replete condition (LB-II)
| PRJNA397765 | ENA
Project description:Salmonella Tn-seq under iron-replete condition (LB-I)
| PRJNA397764 | ENA
Project description:Salmonella Tn-seq under iron-replete condition (LB-III)
| PRJNA397766 | ENA
Project description:Analysis of the Expression Change of RyhB Regulon in RyhB-deleted Mutant Strains. Wild-type Salmonella 14028 vs. three mutant strains (âryhB1, âryhB2, and âryhB1&âryhB2); One condition experiment, Iron-depleted condition by adding 200 µM of 2,2â-dipyridyl (dip).
2010-05-16 | E-GEOD-19813 | biostudies-arrayexpress
Project description:The antimicrobial action of the curing agent NaNO2, which is added as a preservative to raw meat products, depends on its conversion to nitric oxide and other reactive nitrogen species under acidic conditions. In this study, we applied RNA-sequencing to analyze the acidified NaNO2 shock and adaptive response of Salmonella Typhimurium, a frequent contaminant in raw meat. Upon a 10 minute exposure to 150 mg/l NaNO2 in LB pH 5.5 acidified with lactic acid, genes involved in nitrosative stress protection together with several other stress related genes were induced. To the contrary, genes involved in translation, transcription, replication and motility were down-regulated. Induction of stress tolerance and reduction of cell proliferation obviously promote survival under harsh acidified NaNO2 stress. The subsequent adaptive response was characterized by up-regulation of NsrR-regulated genes and iron-uptake systems and down-regulation of genes involved in anaerobic respiratory pathways. Strikingly, amino acid decarboxylase systems, which contribute to acid tolerance, displayed increased transcript levels in response to acidified NaNO2. The induction of systems known to be involved in acid resistance indicates a nitrite mediated increase of acid stress. Transcriptome of Salmonella Typhimurium 14028, treated at OD600 = 0.80-0.85 with 150 mg/l NaNO2 (acidified by lactic acid in the growth medium) for 10 min (shock response) or until an OD600 = 1.45-1.55 is reached (1.5 - 2.0 h, adaptation response), was compared to respective control cultures without NaNO2. Amplified cDNA libraries for sequencing on the SOLiD 5500xl system were prepared from one culture per condition.
2014-08-25 | E-GEOD-57238 | biostudies-arrayexpress
Project description:Staphylococcus lugdunensis is a coagulase-negative Staphylococcus part of the commensal skin flora but emerge as an important opportunistic pathogen. Because iron limitation is a crucial stress during infectious process, we performed phenotypic study and compared proteomic profiles of this species incubated in absence and in presence of the iron chelator 2,2'-dipyridyl (DIP). No modification of cell morphology nor cell wall thickness were observed in presence of DIP. However iron-limitation condition promoted biofilm formation and reduced the ability to cope with oxidative stress (1 mM H2O2). In addition, S. lugdunensis N920143 cultured with DIP was significantly less virulent in the larvae of Galleria mellonella model of infection than that grown under standard conditions. We verified that these phenotypes were due to an iron limitation by complementation experiments with FeSO4. By mass spectrometry after trypsin digestion, we characterized the first iron-limitation stress proteome in S. lugdunensis. Among 1426 proteins identified, 349 polypeptides were differentially expressed. 222 were more and 127 less abundant in S. lugdunensis incubated in iron-limitation condition, and by RT-qPCR, some of the corresponding genes have been shown to be transcriptionally regulated. Our data revealed that proteins involved in iron metabolism and carriers were over-expressed, as well as several ABC transporters and polypeptides linked to cell wall metabolism. Conversely, enzymes playing a role in the oxidative stress response (especially catalase) were repressed. This phenotypic and global proteomic study allowed characterization of the response of S. lugdunensis to iron-limitation. We showed that iron-limitation promoted biofilm formation, but decrease the oxidative stress resistance that may, at least in part, explained the reduced virulence of S. lugdunensis observed under low iron condition.
2020-10-13 | PXD021832 | Pride