Project description:To analysis Mst1r and slpi function as a tumor associated gene, we carried out overexpression analysis of Mst1r or Slpi to Hepa1c1c7 and investigated downstream genes expression by microarray.
Project description:RATIONALE: Studying the genes expressed in samples of tissue from patients with cancer may help doctors identify biomarkers related to cancer.
PURPOSE: This laboratory study is using gene expression profiling to evaluate normal tissue and tumor tissue from patients with colon cancer that has spread to the liver, lungs, or peritoneum.
Project description:Gene expression profiling of SNB19 and U251 glioblastoma cell lines transfected with the FGFR3-TACC3 fusion, FGFR3 wildtype and TACC3 wildtype constructs.
Project description:We used the myoma model in conjunction with gene expression profiling with microarray data as an efficient tool for high throughput analysis and to screen for differentially expressed genes. Our aim was to identify candidates playing an important role in SLPI and/or MMP-promoted tumor invasion by comparing oral carcinoma Ca9-22 cells, which highly express secretory leukocyte protease inhibitor (SLPI) gene, with SLPI-deficient Ca9-22 cells. In oral carcinoma Ca9-22 and SLPI-deficient Ca9-22 (ÎSLPI) samples, gene expression profiling was performed with the Affymetrix Human Genome U133 Plus 2.0 Array. Two arrays for 2 cell lines were analyzed.
Project description:We used the myoma model in conjunction with gene expression profiling with microarray data as an efficient tool for high throughput analysis and to screen for differentially expressed genes. Our aim was to identify candidates playing an important role in SLPI and/or MMP-promoted tumor invasion by comparing oral carcinoma Ca9-22 cells, which highly express secretory leukocyte protease inhibitor (SLPI) gene, with SLPI-deficient Ca9-22 cells.
Project description:This profiling experiment evaluates the global changes in miRNA expression in monocytic cells in response to 10 nM Estradiol treatment. Levels of circulating 17β-estradiol (E2) may influence the progression of many human diseases. We hypothesize that E2 modulates the inflammatory response of circulating innate immune cells through microRNA (miRNA)-based modulation of secretory leucoprotease inhibitor (SLPI), a multifunctional antiprotease with immunomodulatory functions. Monocytes were treated with E2 or control, and differentially expressed miRNAs in monocytes were identified using PCR profiling. Cells were transfected with miRNA mimics or antimiRs and SLPI mRNA and protein levels were quantified by qPCR and ELISA, respectively. Luciferase activity assay was performed using SLPI-3’UTR reporter constructs. ChIP was carried out on monocytes treated with E2. SLPI expression is downregulated, and miR-19 is upregulated in response to E2 in monocytes, via increased MIR17HG promoter activity mediated by c-MYC. Transfection of monocytic cells with premiR-19b reduced SLPI expression. This was abrogated using antimiRs against miR-19b. miR-19b directly binds the SLPI 3’UTR as determined by luciferase activity assay. The data show that E2 decreases expression of SLPI in human monocytic cells, via changes in miRNA expression and further highlights the potential for E2 to modulate key components of innate immunity. Modulation of these miRNAs may offer a new therapeutic approach in the treatment of chronic inflammatory diseases.