Project description:HuMiChip was used to analyze human oral and gut microbiomes, showing significantly different functional gene profiles between oral and gut microbiome. The results were used to demonstarte the usefulness of applying HuMiChip to human microbiome studies.

2014-02-04 | E-GEOD-54290 | biostudies-arrayexpress

RNA sequencing of unmanipulated and saline-injected skin in healthy old adults before and after oral vitamin D3 supplementation

Project description:Vitamin D insufficiency may exacerbate non-specific inflammation observed in older adults. Here, we tested the hypothesis that an inflammatory gene signature present in old skin following saline injection (as model for non-specific needle injury) normalizes after oral vitamin D3 supplementation. To define the saline-induced signature, we compared gene expression in skin biopsies taken six hours after saline injection in old adults (≥65 years) to biopsies from unmanipulated skin. We then assessed signature expression in saline-injected skin of old and young adults (<40 years), and in paired samples of old adults before and after oral vitamin D3 supplementation (6400 IU/day for 14 weeks), where median serum 25-hydroxyvitamin D increased from 43 nmol/L (interquartile range 36-53 nmol/L) to 131 nmol/L (interquartile range 115-147 nmol/L). This submission comprises 112 samples from 57 individuals.

2021-01-02 | E-MTAB-9789 | biostudies-arrayexpress

Age associated differences in microbiome in Rhesus Macaque

Project description:Aging is associated with declining immunity and inflammation as well as alterations in the gut microbiome with a decrease of beneficial microbes and increase in pathogenic ones. The aim of this study was to investigate aging associated gut microbiome in relation to immunologic and metabolic profile in a non-human primate (NHP) model. 12 old (age>18 years) and 4 young (age 3-6 years) Rhesus macaques were included in this study. Immune cell subsets were characterized in PBMC by flow cytometry and plasma cytokines levels were determined by bead based multiplex cytokine analysis. Stool samples were collected by ileal loop and investigated for microbiome analysis by shotgun metagenomics. Serum, gut microbial lysate and microbe-free fecal extract were subjected to metabolomic analysis by mass-spectrometry. Our results showed that the old animals exhibited higher inflammatory biomarkers in plasma and lower CD4 T cells with altered distribution of naïve and memory T cell maturation subsets. The gut microbiome in old animals had higher abundance of Archaeal and Proteobacterial species and lower Firmicutes than the young. Significant enrichment of metabolites that contribute to inflammatory and cytotoxic pathways was observed in serum and feces of old animals compared to the young. We conclude that aging NHP undergo immunosenescence and age associated alterations in the gut microbiome that has a distinct metabolic profile.

2022-02-01 | E-MTAB-10160 | biostudies-arrayexpress

Project description:human-oral sequencingDental caries associated saliva metabolome and its relationship to microbiome alterations in young adults

| PRJNA822496 | ENA

HuMiChip analysis of 86 samples from different body sites

Project description:HuMiChip was used to analyze human oral and gut microbiomes, showing significantly different functional gene profiles between oral and gut microbiome.

2014-02-04 | GSE54290 | GEO

P. pacificus and C. elegans exposed to various pathogens

Project description:This SuperSeries is composed of the following subset Series: GSE36413 : C. elegans young adults : Exposed to Bacillus thuringiensis DB27 vs. E. coli OP50 exposure; 4hours GSE36493: C. elegans young adults: Exposed to Staphylococcus aureus versus exposed to E. coli OP50 : 4 hours GSE36499: C. elegans young adults: Exposed to Serratia marcescens versus exposed to E. coli OP50 : 4 hours GSE36501: C. elegans young adults: Exposed to Xenorhabdus nematophila versus exposed to E. coli OP50 : 4 hours GSE36517: P. pacificus young adults: Exposed to Bacillus thuringiensis DB27 versus exposed to E. coli OP50 : 4 hours GSE36519: P. pacificus young adults: Exposed to Staphylococcus aureus versus exposed to E. coli OP50 : 4 hours GSE36521: P. pacificus young adults: Exposed to Serratia marcescens versus exposed to E. coli OP50 : 4 hours GSE36523: P. pacificus young adults: Exposed to Xenorhabdus nematophila versus exposed to E. coli OP50 : 4 hours Refer to individual Series

2012-03-20 | E-GEOD-36636 | biostudies-arrayexpress

Exploring the microbiome of oral epithelial dysplasia as a predictor of malignant progression

Project description:The objectives of this study were to establish a microbiome profile for oral epithelial dysplasia using archival lesion swab samples to characterize the community variations and the functional potential of the microbiome using 16S rRNA gene sequencing

2022-09-30 | GSE198811 | GEO

The Effects of Benoxacor on the Liver and Gut Microbiome of C57BL/6 Mice

Project description:We report the hepatotoxicity and effects of benoxacor on the gut microbiome following an acute oral exposure.

2021-10-01 | GSE172173 | GEO