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Gene expression profiling of myofibroblasts in young and aged day 5 wound beds

ABSTRACT: Gene expression profiling of myofibroblasts in young and aged day 5 wound beds

PROVIDER: PRJNA415415 | ENA |

REPOSITORIES: ENA

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Gene expression profiling of myofibroblasts in young and aged day 5 wound beds

Project description:Mesenchymal cells were isolated from day 5 dorsal skin wound beds of 7-weeks old and >24-months old using FACS. This experiment describes multiple unique subsets of wound bed myofibrolbasts capable of contributing to tissue repair.

2018-11-06 | GSE105790 | GEO

Gene expression profiling of adipocyte precursor cells (AP) nonwounded and day 5 wound beds

Project description:APs were isolated from naïve skin and day 5wounds from dorsal skin wound beds of 7-9 weeks old using FACS. This experiment describes changes in AP gene expression associated with injury and subsequent tissue repair.

2018-11-06 | GSE105788 | GEO

Gene expression profiling of CD301b+ macrophages and F4/80 negative immune cells from day 5 mouse wound beds

Project description:Cells were isolated from day 5wounds from dorsal skin wound beds of 7-9 weeks old using FACS. This experiment describes the gene expression profile associated with different immune cell subsets during tissue repair.

2018-11-06 | GSE105789 | GEO

Role of stromal fibroblasts and myofibroblasts in corneal wound healing

Project description:Transparent avascular cornea providing two third refraction to the eye. Restoration of corneal transparency and clear vision in a traumatic eye involves the action of many cytokines and signaling pathways. Out of several factors, stromal keratocytes/fibroblasts (CSFs) play a central role in corneal repair and wound healing. Post trauma, keratocytes/fibroblasts produce myofibroblasts to facilitate wound repair by synthesizing and secreting large extracellular matrix components, collagens, and alpha-smooth muscle actin stress fibers. This study aimed to perform RNASeq data analysis and pathway enrichments to gain a better understanding of gene regulation in corneal fibroblasts and myofibroblasts in corneal wound repair.

2024-03-11 | GSE260476 | GEO

scRNA-Seq for mouse wounded and non-wounded samples

Project description:Lineage negative (CD45- & CD31-) cells were isolated from uninjured skin and day 5 dorsal skin wound beds of 7-week-old AdipoqCre; mT/mG mice using FACS. This experiment describes multiple subsets of wound bed myofibroblasts and identifies that Adipoq-traced cells contribute to the myofibroblast pool in wound beds.

2020-04-14 | GSE140512 | GEO

Gene expression profiling of adipocyte precursor cells (AP) nonwounded and day 5 wound beds

Project description:Gene expression profiling of adipocyte precursor cells (AP) nonwounded and day 5 wound beds

| PRJNA415412 | ENA

Changes in miRNA expression during wound healing in mouse aged skin

Project description:To identify the candidate miRNAs that might compromise wound healing and contribute to the age-associated delay in wound repair, global miRNA profiling was performed in mouse back telogen skin of young (8-week-old) and aged (2-year-old) animals.

2017-03-25 | GSE97034 | GEO

Fat cells regenerate from myofibroblasts during wound healing

Project description:We report transcriptomes of myofibroblasts from mouse skin wounds. Myofibroblasts were FACS sorted as Zombie-neg;tdTomato-hi cells from Sm22-Cre;TdTomato mice. We identified and analyzed 4,120 differentially expressed transcripts across four post-wounding time points, day 12, day 15, day 21 and day 26.

2016-12-07 | GSE84256 | GEO

Gene expression profiling of skin adipocyte and adipocyte derived cells in mice 5 days after injury

Project description:Cells were isolated from day 5wounds from dorsal skin wound beds of 7-9 weeks old using FACS. This experiment describes the gene expression profile associated with mature adipocytes, adipocyte-derived cells and myofibroblasts during tissue repair.

2020-02-14 | GSE126514 | GEO

ANT2 accelerates cutaneous wound healing in aged skin by reverse senescence

Project description:An effective healing response is critical to healthy aging. Thus, the connection of regeneration and aging is needed to understand the complicated age-related healing process. Energy metabolism has been a common hallmark of both studies. In recent years, it become an emerging factor of skin homeostasis. Adenine nucleotide translocase-2 (ANT2) is a known cell proliferation marker and mediator of ATP import into mitochondria for energy homeostasis. Although energy homeostasis and the maintenance of mitochondrial function are critical for wound healing, the role of ANT2 in wound healing has not been elucidated. We found that ANT2 expression decreased during aging in mouse skin as well as during cellular senescence. Interestingly, overexpression of ANT2 in aged mouse skin promoted the healing of full-thickness cutaneous wounds. In addition, upregulation of ANT2 in replicative senescent human diploid dermal fibroblasts (HDFs) induced cell proliferation and migration, which are critical for the wound healing process. Furthermore, overexpression of ANT2 increased ATP production rate by activating the glycolysis pathway and also increased mitophagy, both of which are involved in energy homeostasis. Notably, ANT2-mediated upregulation of HSPA6 in aged HDFs inhibited the expression of pro-inflammatory genes that mediate cellular senescence and mitochondrial damage. This study demonstrates a new physiological role of ANT2 in skin wound healing via regulation of cell proliferation, energy homeostasis, and inflammation. Thus, our study links energy metabolism to skin homeostasis and identifies a genetic factor for improving wound healing with aging model.

2022-11-21 | GSE206141 | GEO

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