Project description:Here we used human cortical brain organoids to probe the longitudinal impact of GSK3 inhibition through multiple developmental stages. Chronic GSK3 inhibition increased the proliferation of neural progenitors and caused massive derangement of cortical tissue architecture. Cortical organoids were differentiated as previously described (Paşca et al., 2015, doi: 10.1038/nmeth.3415.).Chronic GSK3 inhibition was performed by adding CHIR99021 (Merck SML1046) to the medium at day 0 (1 microM) and kept throughout the differentiation process until reaching the respective collection timepoints (day 18, day 50, day 100).

2020-06-04 | E-MTAB-8325 | biostudies-arrayexpress

DNA Methyaltion of Human Cortical Organoids

Project description:DNA Methyaltion of Human Cortical Organoids

| PRJNA632019 | ENA

22q11.2 deletion syndrome human cortical organoids

Project description:22q11.2 deletion syndrome human cortical organoids

| PRJNA595613 | ENA

DNA Methyaltion of Human Cortical Organoids

Project description:Assesing DNA methylation patterns of human cortical organoids during long term (>600 days) maturation.

2020-12-25 | GSE150122 | GEO

Modeling Focal Cortical Dysplasia (FCD) using cortical organoids

Project description:We generated cortical organoids from four FCD patients. To generate cortical organoids, we used induced pluriplotent stem cells (iPSCs) obtained from skin biopsy from these FCD selected patients and healthy controls. We extrated RNA samples from the cortical organoids to do customized panel of gene expression. Gene expression using NanoString Human Neuropathology Panel from four FCD patients and four controls

2022-01-01 | GSE174605 | GEO

22q11.2 deletion syndrome human cortical organoids

Project description:We derived iPSC from individuals carrying a 22q11.2 deletion and controls and differentiated them into 3D cortical organoids.

2020-07-08 | GSE142041 | GEO

Vascularized human cortical organoids (vOrganoid) model cortical development in vivo

Project description:Vascularized human cortical organoids (vOrganoid) model cortical development in vivo

| PRJNA542649 | ENA