Project description:Autophagy is a lysosomal degradation pathway critical for maintaining cellular homeostasis and viability, and is predominantly regarded as a rapid and dynamic cytoplasmic process. To improve our understanding of the transcriptional and epigenetic events associated with autophagy, we performed genome-wide transcriptomic and epigenomic profiling after nutrient deprivation in human wildtype and autophagy-deficient cells. We observed that nutrient deprivation leads to the transcriptional induction of numerous autophagy-associated genes. These transcriptional changes are reflected at the epigenetic level (H3K4me3, H3K27ac, and H3K56ac) and are independent of autophagic flux.
Project description:Autophagy is a lysosomal degradation pathway critical for maintaining cellular homeostasis and viability, and is predominantly regarded as a rapid and dynamic cytoplasmic process. To improve our understanding of the transcriptional and epigenetic events associated with autophagy, we performed genome-wide transcriptomic and epigenomic profiling after nutrient deprivation in human wildtype and autophagy-deficient cells. We observed that nutrient deprivation leads to the transcriptional induction of numerous autophagy-associated genes. These transcriptional changes are reflected at the epigenetic level (H3K4me3, H3K27ac, and H3K56ac) and are independent of autophagic flux.
Project description:Transcriptional profiling showed that adaptation to nutrient and oxygen deprivation condition is associated with widespread transcriptional reprogramming resulting in the induction of glycolysis and autophagy and repression of the TCA cycle and biosynthesis
Project description:Transcriptional profiling showed that adaptation to nutrient and oxygen deprivation condition is associated with widespread transcriptional reprogramming resulting in the induction of glycolysis and autophagy and repression of the TCA cycle and biosynthesis
Project description:To thoroughly characterize the diapaused blastocyst induced by ovariectomy, maternal starvation, and nutrient deprivation, we executed RNA-sequencing analyses for ND blastocysts at E4.5, DIA, Sta, K-PC, and K+0.02 blastocysts at E5.5.
Project description:Transcriptional profiling showed that adaptation to nutrient and oxygen deprivation condition is associated with widespread transcriptional reprogramming resulting in the induction of glycolysis and autophagy and repression of the TCA cycle and biosynthesis