Project description:In recent years, there is a growing interest in the role of PPARγ agonists in skin biology. Specifically, we have previously shown that agonistic PPARγ modulators affect hair follicle growth and function. PPARγ-mediated signalling has also been implicated in the regulation of mitochondrial energy metabolism in several tissues (adipose and brain), but how PPARγ-stimulation affects mitochondrial biology in the skin is still unknown. In this study, we aimed to explore the effects of PPARγ-stimulation in organ cultured human hair follicles.
Project description:Telogen (resting phase) hair follicles are more radioresistant than anagen (growth phase) ones. Irradiation of BALB/c mice in the anagen phase with γ-rays at 6 Gy induced hair follicle dystrophy, whereas irradiation in the telogen phase induced the arrest of hair follicle elongation without any dystrophy after post-irradiation depilation. In contrast, FGF18 was highly expressed in the telogen hair follicles to maintain the telogen phase and also the quiescence of hair follicle stem cells. Therefore, the inhibition of FGF receptor signaling at telogen induced the dystrophy after post-irradiation depilation. In addition, the administration of recombinant FGF18 suppressed cell proliferation in the hair follicles and enhanced the repair of radiation-induced DNA damage, so FGF18 protected the anagen hair follicles against radiation damage to enhance hair regeneration. Moreover, FGF18 reduced the expression of cyclin B1 and cdc2 in the skin and FGF18 signaling induced G2/M arrest in the keratinocyte cell line HaCaT, although no obvious change of the expression of DNA repair genes was detected by DNA microarray analysis. These findings suggest that FGF18 signaling for the hair cycle resting phase causes radioresistance in telogen hair follicles by arresting the proliferation of hair follicle cells.
Project description:Investigation of Gene Expression Profiling in Unstaged Head Hair Follicles Plucked from Men and Women Keywords: Gene Expression Profiling of Normal Hair Follicles
Project description:rationale: comparison of gene expression profiles in wildtype and Foxn1::dnFGFR2-IIIb transgenic hair follicles; identification of targets that mediate the effects seen in transgenic hair follicles; results: as already suggested by the phenotype, the molecular abnormalities appear to be restricted to the hair shaft medulla; Igfbp5 is an important mediator of transgene-dependent effects
Project description:Pla2g2e is dominantly expressed in hair follicles. Microarray gene profiling showed modest changes in the steady-state expression of a subset of skin genes.
Project description:To compare the effects of spermidine, key polyamine, on the gene expression profile of organ cultured human hair follicles Vehicle treated Vs. 0.5 µM spermidine treatment. Two control samples, two spermidine treated samples
Project description:The human hair follicle is not only regulated, but is a source of transcription factors, hormones, neurohormones etc. which are crucial in regulating its growth. We used microarrays to determine the early response of microdissected human hair follicles to an osteopontin-derived peptide.
Project description:Hepatocyte growth factor (HGF) is a well-known promoter of cell proliferation, morphogenesis, migration, angiogenesis and pigmentation. To characterize its effects on human scalp dissected hair follicles, we performed gene expression profiling on RNA isolated from entire hair follicles, comparing the effects of 6hr HGF treatment versus control. 183 genes were differentially up-/down-regulated compared to vehicle control. These genes were involved in a number of different biological pathways suggesting that HGF can have various effects upon human hair follicle function.
Project description:Investigation of Gene Expression Profiling in Unstaged Head Hair Follicles Plucked from Men and Women Experiment Overall Design: Total RNA was extracted from 1-3 unstaged follicles plucked from the scalp of each of 36 adult human volunteers. The average quantifiable yield of RNA per follicle was 112.5 ng. Ten samples were selected, based on ribosomal ratio, relative integrity number and total yield, for expression profiling. By preamplifying the extracted RNA (starting with as little as 30 ng), sufficient labeled RNA was generated to conduct Afymetrix-based gene expression analysis.