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ALV-J and REV synergistically increase the accumulation of miRNAs

ABSTRACT: ALV-J and REV synergistically increase the accumulation of miRNAs

PROVIDER: PRJNA429641 | ENA |

REPOSITORIES: ENA

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ALV-J and REV synergistically increase the accumulation of miRNAs

Project description:The aim of this study was to identify the miRNAs in CEF in response to synergistic infection of ALV-J and REV, as well as attempt to reveal the mechanism underlying pathogenesis and immune suppression symptoms.

2018-01-11 | GSE109105 | GEO

RNA-Seq for ALV-J-susceptible and ALV-J-resistant chickens

Project description:We bed ALV-J-susceptible and ALV-J-resistant chickens. In this work, we find the different gene expression between ALV-J-susceptible and ALV-J-resistant chickens

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MeDIP-Seq for ALV-J-susceptible and ALV-J-resistant chickens

Project description:We bed ALV-J-susceptible and ALV-J-resistant chickens. In this work, we find the difference DNA methylation states between ALV-J-susceptible and ALV-J-resistant chickens

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RNA-seq analysis of miRNAs expression in chciken ALV-J-infected primary monocyte-derived macrophages (MDM) and uninfected control.

Project description:Purpose: The goals of this study are to investigate the differentially expressed miRNAs between ALV-J-infected primary monocyte-derived macrophages (MDM) and uninfected control by Illumina deep sequencing. Methods:Total RNA from two ALV-J-infected MDM (designated: J3h_1, J3h_2, J36h_1 and J36h_2) and two uninfected MDM samples (designated: NC3h_1, NC3h_2, NC36h_1 and NC36h_2) was isolated by TRIzol following the manufacturer’s instruction at 3 h post infection (hpi) and 36 hpi. RNA samples of two individuals within each group were pooled with equal amounts, and then were subjected to Illumina deep sequencing by Illumina Hiseq 2000. Results: compared to the uninfected MDM, we identified 13 significant up-regulated miRNAs and 2 significant down-regulated miRNAs in ALV-J infected MDM at 3 hpi, and 6 significant up-regulated miRNAs and 2 significant down-regulated miRNAs in ALV-J infected MDM at 36 hpi. Conclusions: Our results suggest that DE miRNAs involved in the immune response induced by ALV-J infection in MDM at 3 hpi. In addition, only 25 miRNAs-target DEGs were identified in MDM with ALV-J infection at 36 hpi, and these target DEGs can’t be significantly enriched in any GO terms and KEGG pathway..

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Reticuloendotheliosis virus and avian leukosis virus subgroup J synergistically increase the accumulation of exosomal miRNAs.

Project description: Not available

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Project description:In order to found the target genes of ALV-miR-p19-01 which encoded by ALV-J, we used RNA-pull down and RNA-seq. The resulted showed there were 917 potential target genes of ALV-miRNA-p19-01 had been identified

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2017-10-04 | E-MTAB-5135 | biostudies-arrayexpress

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