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DNA methylation Profiling in Kawasaki disease (KD): DNA methylation profile in healthy control subjects, acute-phase KD subjects and convalescent-phase KD subjects

ABSTRACT: DNA methylation Profiling in Kawasaki disease (KD): DNA methylation profile in healthy control subjects, acute-phase KD subjects and convalescent-phase KD subjects

PROVIDER: PRJNA430873 | ENA |

REPOSITORIES: ENA

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Exon Level Expression Profiling in Kawasaki disease (KD): gene expression profile in healthy control subjects, acute-phase KD subjects and convalescent-phase KD subjects

Project description:Exon Level Expression Profiling in Kawasaki disease (KD): gene expression profile in healthy control subjects, acute-phase KD subjects and convalescent-phase KD subjects

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DNA methylation Profiling in Kawasaki disease (KD): DNA methylation profile in healthy control subjects, acute-phase KD subjects and convalescent-phase KD subjects

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Exon Level Expression Profiling in Kawasaki disease (KD): gene expression profile in healthy control subjects, acute-phase KD subjects and convalescent-phase KD subjects

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Global gene expression profiling identifies new therapeutic targets in acute Kawasaki disease

Project description:Transcriptional profile in Kawasaki patients at acute and convalescent phase with different clinical outcomes were investigated. To gain further insight into the molecular mechanisms underlying KD, we investigated the acute and convalescent whole blood transcriptional profiles of 171 KD subjects and compared them with the transcriptional profiles of pediatric patients with confirmed bacterial or viral infection, and with healthy control children. We also investigated the transcript abundance in patients with different intravenous immunoglobulin treatment responses and different coronary artery outcomes.

2014-12-06 | E-GEOD-63881 | biostudies-arrayexpress

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Kawasaki Disease and Other Febrile Illnesses

Project description:ABSTRACT Background. Acute Kawasaki disease (KD) is difficult to distinguish from other acute rash/fever illnesses, in part because the etiologic agent(s) and pathophysiology remain poorly characterized. As a result, diagnosis and critical therapies may be delayed. Methods. We used DNA microarrays to identify possible diagnostic features of KD. We compared gene expression patterns in the blood of 23 children with acute KD and 18 age-matched febrile children with three illnesses that resemble KD. Results. Genes associated with platelet and neutrophil activation were expressed at higher levels in KD patients than in patients with acute adenovirus infections or systemic adverse drug reactions but not in patients with scarlet fever; genes associated with B cell activation were also expressed at higher levels in KD patients than in controls. A striking absence of interferon-stimulated gene expression in the KD patients was confirmed in an independent cohort of KD subjects. We successfully predicted the diagnosis in 21 of 23 KD patients and 7 of 8 adenovirus patients using a set of 38 gene transcripts. Conclusions. These findings provide insight into the molecular features that distinguish KD from other febrile illnesses, and support the feasibility of developing novel diagnostic reagents for KD based on the host response. A disease state experiment design type is where the state of some disease such as infection, pathology, syndrome, etc is studied. Disease State: One of Kawasaki Disease (KD) or control (C) of Scarlet fever (C-sf), adenovirus infection (C-ai) or drug reaction (C-dr) disease_state_design

2010-06-24 | E-GEOD-15297 | biostudies-arrayexpress

Kawasaki Disease and Other Febrile Illnesses

2009-08-12 | GSE15297 | GEO

Human PBMNCs: Control vs. Patients with Kawasaki disease

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2010-09-04 | E-GEOD-17975 | biostudies-arrayexpress

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Project description:Peripheral blood samples were taken from a total of 48 individuals (24 KD patients and 24 normal controls) and subjected to access DNA CpG methylation state using Infinium HumanMethylation450 BeadChips. This array consists of probes to determine the site-specific methylation status of more than 450K CpG loci across the human genome. Approximately 500 ng of bisulfate-converted DNA (BCD) was used per BeadChip for hybridization in accordance with the protocol and instructions of Infinium HD methylation. After completing the whole-genome amplification step and then controlled enzymatic end-point fragmentation, BCD samples were precipitated and re-suspended for hybridization for 16 h at 48°C. During this process, the DNA molecules are annealed to two bead types with methylated loci (C)-specific and unmethylated loci (T)-specific oligomers. Afterward, the non-hybridized or mis-hybridized DNA was washed away, followed by single-base extension using 2,4-dinitrophrnol- and biotin-labeled ddNTPs and the annealed BCD as templates.

2016-12-31 | GSE84624 | GEO

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