Project description:Dieldrin is a pesticide with a suspected impact on chromatin. Here, we have exposed Jurkat cells to dieldrin for 30 min. and examined by next generation sequencing the impact on transcription. This impact was compared to that of DMSO (solvent of the dieldrin) and that of PMA.
Project description:In this research, we use DNA microarray analysis to clarify the gene expression responses in Jurkat cells after Tamoxifen treatment. Jurkat cells are a dexamethasone-resistant cell line derived from a T-cell Acute Lymphoblastic Leukaemia sample in relapse
Project description:Cut&Run analysis was performed in TAL1-FKBP12 Jurkat cells to analyze DNA bindings of GATA3 and RUNX1 after DMSO or dTAG-13 treatment.
Project description:To identify novel target genes regulated by LEF1 transcription factor in T-ALL, we examined changes in the genome-wide gene expression profile by microarray in siRNA mediated LEF1 knockdown Jurkat cells. We used microarray to determine the differential gene expression in LEF downregulated Jurkat cells by LEF1 siRNA.
Project description:Jurkat cell lines were generated to stably express CD46 protein expressing either cytoplasmic tail 1 or 2 (BC1 or BC2). The transcription profile of these unactivated stable lines were compared with unactivated Jurkat cells.
Project description:Jurkat T cell line was transfected with SIV Nef controlled by an unducible promoter system. Nef was induced by addition of 10 micromolar pronasterone A and gene expression values were determined after 24 hrs. Control Jurkat cells were untransfected and treated with 10 micromolar pronasterone A and gene expressio values were determined after 24 hrs. Keywords: SIV Nef, Jurkat, pronasterone A
Project description:The LEDGF transcript from the PSIP1 gene was knocked down in Jurkat cells using RNAi technology. The resulting Jurkat-derived cell line (Jurkat-siJK2) was compared to a control cell line (wild type Jurkat) using microarray analysis. Genes identified as being modulated by LEDGF were preferential targets of HIV integration. Keywords: effects of gene knockdown