Project description:In this study, we tested the hypothesis that restoration of miR-30a expression in WAT would improve peripheral insulin sensitivity. Exogenous miR-30a expression in subcutaneous WAT depots of obese mice improved insulin sensitivity, decreased ectopic liver fat deposition, and reduced WAT inflammation compared to matched controls. We identified the target genes and pathways of miR-30a using RNA-Seq technology
Project description:RNA-sequencing was performed to gain insight into the transcriptome-wide molecular changes induced by miR-30a-5p or miR-30a-3p over-expression in lung adenocarcinoma cells. Following transfection of miR-30a-5p or miR-30a-3p miRNA mimic or control RNA, RNA-sequencing was performed. This high-throughput data revealed elevated expression of both miR-30a-5p and miR-30a-3p reduced the expression of genes and pathways known to induce proliferation and movement in lung adenocarcinoma cells.
Project description:To study effect of niacin bound chromium in changing genetic profile of subcutaneous fat from diabetic mice Keywords: nutritional supplemetation, obesity
Project description:Human miR-30a-3p was knocked-down in HepG2 using siRNA duplex against the miRNA precursor, and compared to control cells on GeneChip to identify the target transcripts against miR-30a-3p in vivo.
Project description:Gene expression from epidydimal fat pad of wild type C57BL6/J and Apoliprotein E (EKO) mice on C57BL6/J mice Whole tissue of wild type and EKO mice fed with normal and high fat diet (3 and 4 biological replicates)
Project description:The goal was to identify genes targeted by miR-30a. Keywords: Transfection of cultured cells with antisense oligonucleotide RNA was isolated from BMELs tranfected with a locked nucleic acid (LNA) antisense oligonucleotide to miR-30a or with a scrambled control oligo.
Project description:ATAC-seq on human mesenteric fat pad For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf
Project description:ChIP-seq on human omental fat pad For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf