Project description:We catalogued and quantified the transcripts in livers of wild-type and Aag-deficient mice that had been exposed to the model alkylating agent methyl methanesulfonate (MMS). Gene expression analysis was performed for samples harvested 6 hours post-MMS treatment.
Project description:We analyzed the proteomic changes that occur in a Fbxl4 knock-out 1-year mouse. We analyzed Liver tissue using a 6-plex TMT approach (3 KO and 3 controls). Since we observed a global decrease in mitochondrial proteins, we also explored mitoproteome changes in different tissues (liver, kidney and heart) using a label-free approach. Finally, we also did a 6-plex TMT to analize the proteomic changes in 3 patient-derived fibroblast lines compared to 3 control lines and correlated them with the results obtained in the mouse model. All together, these experiments revealed that Fbxl4 deficiency leads to a decreased mitochondrial content without major changes in mitochondria itself, pointing to an increased turnover.
Project description:Mass spectrometry-based whole proteome analysis of parental and RFX7 knock-out U2OS cells treated with 10 µM Nutlin-3a or DMSO solvent control. Ten biological replicates were used.
Project description:To explore the circadian regulations of Bmal1, we examined the transcriptome changes in mouse livers upon Bmal1 knock out at two circadian time points, CT0 and CT12.
Project description:the gene expression profiling results provide important information for the genes regulated by crosstalk between Shp2 and Pten mediated signal pathways Total RNA was extracted from CD71mid Ter119high erythroblasts isolated from the bone marrow of wide type, Shp2 knock-out, Pten knock-out and double knock-out mice