Ontology highlight
ABSTRACT:
PROVIDER: PRJNA474385 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Allicin is a natural extract from garlic that has been used as an antibiotic alternative in livestock production. Limited knowledge is known about the effects of allicin on gut microbiota and tissue. The aim of this study was to investigate the effects of allicin on the expression of ileal miRNAs and the tissue-associated total bacterial density in pre-weaned calves. Different doses of allicin (0 mg, 0.5 mg, 5 mg, 50 mg and 500 mg) were used to treat in vivo ileal loop tissues in pre-weaned calves for different periods (1 day, 3 days and 12 days). A total of 15 miRNAs showed allicin dose-dependent differential expression patterns. Four miRNAs (miR-132, miR-423-5p, miR-429 and miR-6529a) were upregulated in 50 mg or 500 mg treated gut tissues, while 11 miRNAs (miR-190a, miR-193a-3p, miR-196a, miR-196b, miR-2285aa, miR-2285o, miR-2887, miR-2904, miR-409a, miR-6523a and miR-96) were downregulated in 50 mg or 500 mg treated gut tissues. Functional analysis of differentially expressed mRNAs showed that the upregulated mRNAs regulate functions that may be involved in “disrupted microtubule function”, and the downregulated miRNAs may be associated with the “inhibited leukocyte transendothelial migration” and “promoted cell phagocytosis”.
2016-11-01 | GSE74503 | GEO
Project description:Serotonin is a monoamine that regulates processes such as energy balance and immune function. Manipulating this pathway in growing dairy calves could promote growth and development by modulating functions and signaling pathways within key organs. In this study, we characterized the adipose and muscle transcriptome of pre-weaned calves with increased serotonin bioavailability through the elucidation of differentially expressed genes.
2020-06-05 | GSE151781 | GEO
Project description:Calves are highly susceptible to gastrointestinal infection with Cryptosporidium parvum (C. parvum), which can result in watery diarrhea and eventually death or impaired development. With little to no effective therapeutics, understanding the host’s microbiota and pathogen interaction at the mucosal immune system has been critical to identify and test novel control strategies. We used an experimental model of C. parvum challenge in neonatal calves to describe the clinical signs and mucosal innate immune and microbiota hallmarks in the ileum and colon during cryptosporidiosis and investigated the impact of supplemental colostrum feeding on C. parvum infection. The C. parvum challenged calves experienced clinical signs including pyrexia and diarrhea 5 days post challenge. These calves showed ulcerative neutrophil ileitis with a proteomic signature driven by inflammatory effectors, including reactive oxygen species and myeloperoxidases. Colitis was also noticed with an aggravated mucin barrier depletion and lack of full filled mucin granule in goblet cells. The C. parvum challenged calves also displayed a pronounced dysbiosis with a high prevalence of Clostridium species (spp.) and number of exotoxins, adherence factors, and secretion systems related to Clostridium spp. and other enteropathogens, including Campylobacter spp., Escherichia sp., Shigella spp., and Listeria spp. Daily supplementation with a high-quality bovine colostrum product mitigated some of the clinical signs and modulated the gut immune response and concomitant microbiota to a pattern more similar to that of healthy unchallenged calves.
2023-04-27 | PXD040269 | Pride
Project description:Acidification of colostrum affects the fecal microbiota of pre-weaned dairy calves
| PRJNA843323 | ENA
Project description:In order to test the development of gastrointestinal tract (GIT) in pre-weaned cavles, the GIT tissues were collected from day 0, day 7, day 21 and day 42 calves. RNA-seq was used to measure the transcriptome profiles. The RNA-seq analysis revealed the fast development of small intestine and rumen tissue during the first week after birth.
2016-05-01 | GSE74329 | GEO
Project description:Maternal nutrition during gestation can cause epigenetic effects that translate to alterations in gene expression in offspring. This 2-year study employed RNA-sequencing technology to evaluate the pre- and post-vaccination muscle transcriptome of early-weaned Bos indicus-influenced beef calves born from dams offered different supplementation strategies from 57 ± 5 d prepartum until 17 ± 5 d postpartum.
2021-03-03 | GSE168091 | GEO
Project description:The objectives of the study were to use RNA-Seq to examine the effect of (i) breed and (ii) gradual weaning, on the whole blood mRNA transcriptome of artificially reared Holstein-Friesian and Jersey calves. The calves were gradually weaned over 14 days (day (d) -13 to d 0) and mRNA transcription was examined one day before gradual weaning was initiated (d -14), one day after weaning (d 1) and 8 days after weaning (d 8). RNA-seq analysis was carried out on RNA extracted from whole blood. Gradual weaning had no effect on gene expression (P>0.05).There were 550 differentially expressed genes at a false discovery rate of 10% and with a â¥1.5-fold change, between Holstein-Friesian and Jersey calves on d -14, 490 on d 1, and 411 on d 8. GOseq/KEGG pathway analysis showed that the cytokine-cytokine receptor interaction pathway and the neuroactive ligand-receptor interaction pathway were over-represented between breeds on all days (P<0.01; Qâ¤0.1). These results demonstrate that the gradual weaning practiced here does not compromise the welfare of artificially-reared dairy calves, evidenced by the lack of expression changes in any genes in response to gradual weaning. These data also suggest differences in cell signalling and immune responses between breeds. Eight Holstein-Friesian and eight Jersey bull calves were group housed indoors and individually fed milk replacer and concentrate using an automatic feeder. Calves were gradually weaned by reducing milk-replacer from 6 litres to 0 litres over 14 days (d) (d -13 to d 0). Calves were blood sampled on d -14, 1, and 8, relative to weaning (d 0). RNA-seq analysis was carried out on RNA extracted from whole blood.
2016-08-19 | E-GEOD-76841 | biostudies-arrayexpress