Project description:We measured protein translation (by ribosome profiling) and RNA levels (by polyA-enriched RNA-seq) in Cryptococcus neoformans strain H99 and Cryptococcus neoformans strain JEC21. This is the first transcriptome-wide map of translation in this species complex.
Project description:Comparison of transcriptional profiles of WT Cryptococcus neoformans (H99) and strain CM126 (pRPL2b-GAT201) which overexpresses the transcription factor GAT201 using a ribosomal protein promoter Keywords: Genetic modification
Project description:Invasive fungal infections (IFIs) are difficult to treat. Few effective antifungal drugs are available and many have problems with toxicity, efficacy and drug-resistance. To overcome these challenges, existing therapies may be enhanced using more than one agent acting in synergy. Previously, we have found amphotericin B (AMB) and the iron chelator, lactoferrin (LF), were synergistic against Cryptococcus neoformans and Saccharomyces cerevisiae. This study investigates the mechanism of AMB+LF synergy using RNA-seq in Cryptococcus neoformans H99.
Project description:Log phase of Cryptococcus neoformans strain H99 was exposed to amphotericin B (1.5μg/ml) or vehicle (control) for 3h. Transcriptome of drug treated cells were compared to no treatment cells.
Project description:Approximately 1 million cells of Cryptococcus neoformans lab strain H99 were spread on YPD plate supplmemented with 3ug/ml amphotericin B. Randomly 30 adaptors (TJ1832 - TJ1861) were chosen. The parent and all the 30 adaptors were sequenced.
Project description:Cryptococcus neoformans interactions with murine macrophages are critical for disease. In this project we analyzed fungal proteins which were co-purified with murine host proteins after interaction. H99 C. neoformans was opsonized with mAb 18B7 and addedd to murine macrophages. Then murine cells were lysed and cell extracts submitted to proteomics.
Project description:Cryptococcus neoformans lab strain H99 was exposed to 0.0625ug/ml and 0.5ug/ml tunicamycin for 3h. Transcritpomes were compared to no drug treated cells.
Project description:Cryptococcus neoformans lab strain H99 was used to obtain brefeldin A adaptors. Transcriptomes of 4 adaptors, each bearing a unique aneuploid, were compared to H99.
Project description:We exposed Cryptococcus neoformans lab strain H99 to tunicamycin and obtained some adaptors. We did whole genome sequencing of these adaptors as well as the parent.