Project description:Genome-wide mapping of proteinM-bM-^@M-^SDNA interactions is essential for a full understanding of transcriptional regulation. A precise map of binding sites for transcription factors, core transcriptional machinery is vital for deciphering the gene regulatory networks that underlie various biological processes. Chromatin immunoprecipitation followed by sequencing (ChIPM-bM-^@M-^Sseq) is a technique for genome-wide profiling of DNA-binding proteins. However, our conventional ChIPM-bM-^@M-^Sseq occasionally gives wider peaks which might be due to overlapping binding sites of two or more transcription factors. Therefore, to improve the resolution of our conventional ChIPM-bM-^@M-^Sseq which have DNA-protein footprint of ~100 bp, we decreased the size of DNA-protein footprint to ~ 50 bp by DNaseI digestion of whole cell extract (WCE). ChIP-seq for Twist transcription factor in Drosophila embryos
Project description:Deciphering H3K4me3 Broad Domains Associated With Gene Regulatory Networks and Conserved Epigenomic Landscapes in the Human Brain [RNA-Seq]
Project description:Deciphering H3K4me3 Broad Domains Associated With Gene Regulatory Networks and Conserved Epigenomic Landscapes in the Human Brain [ChIP-Seq]
Project description:This is the first study deciphering the global regulatory network that drives human somatic cells during epigenetic rewiring towards the pluripotent state.
Project description:This is the first study deciphering the global regulatory network that drives human somatic cells during epigenetic rewiring towards the pluripotent state.
Project description:This is the first study deciphering the global regulatory network that drives human somatic cells during epigenetic rewiring towards the pluripotent state.
Project description:This is the first study deciphering the global regulatory network that drives human somatic cells during epigenetic rewiring towards the pluripotent state.
