Barley
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ABSTRACT:
PROVIDER: PRJNA485436 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:In this study, possible adverse effects of transgene expression in field-grown barley were assessed in relation to the influence of genetic background and to the impact of interaction with arbuscular mycorrhiza fungi. The deposited microarray data originate from a parallel transcript profiling, metabolome profiling and metabolic fingerprinting approach with the wild type cultivars Golden Promise (GP) and Baronesse (B) as well as barley transgenics with i) seed-specific expression of (1,3-1,4)-ß-glucanase (GluB) being introgressed from the Golden Promise (GP) into the cultivar Baronesse (B) and ii) ubiquitous expression of codon-optimized Trichoderma harzianum endochitinase (ChGP). Our conclusion from the results is that cultivar-specific differences and even the presence of few introgressed alleles exceed the effects on transcriptome and metabolome caused by transgene expression. Mycorrhization was shown to have more prominent effects on the metabolome than on the transcriptome.
2010-03-03 | GSE19296 | GEO
Project description:In this study, possible adverse effects of transgene expression in field-grown barley were assessed in relation to the influence of genetic background and to the impact of interaction with arbuscular mycorrhiza fungi. The deposited microarray data originate from a parallel transcript profiling, metabolome profiling and metabolic fingerprinting approach with the wild type cultivars Golden Promise (GP) and Baronesse (B) as well as barley transgenics with i) seed-specific expression of (1,3-1,4)-ß-glucanase (GluB) being introgressed from the Golden Promise (GP) into the cultivar Baronesse (B) and ii) ubiquitous expression of codon-optimized Trichoderma harzianum endochitinase (ChGP). Our conclusion from the results is that cultivar-specific differences and even the presence of few introgressed alleles exceed the effects on transcriptome and metabolome caused by transgene expression. Mycorrhization was shown to have more prominent effects on the metabolome than on the transcriptome. Hordeum vulgare plants were cultivated in the field in 2007 at Giessen Experimental station (165m elevation over NN, 1540h sunshine and 650mm precipitation per year in average) in replicated blocks, each consisted of eight 0.8 m × 0.5 m plots in which genotypes were randomly distributed and half of the plots were pretreated with Amykor® (containing the arbuscular fungi Glomus intraradices and Glomus mosseae). Ten pools of leaf material of at least 10 leaves per pool were sampled in the middle of the light period four months after planting. Two replicates of each genotype were subjected to microarray analysis.
2010-03-02 | E-GEOD-19296 | biostudies-arrayexpress
Project description:Wild type Golden Promise barley and the frost tolerant TaCBF14 and TaCBF15 transgenic barley lines were grown in plant growth chamber in the Phytotron of ELKH ATK Agricultural Institute, Martonvasar, Hungary. After three weeks in control conditions, plants were treated by short-term cold-treatment. Samples were collected under control condition and after the short-term cold-treatment too.
2021-11-10 | E-MTAB-10781 | biostudies-arrayexpress
Project description:We measured mRNA abundance in the seedling leaves of the barley genotypes Golden Promise and Morex and in F1 hybrids generated using either Golden Promise as a maternal genotype and Morex as paternal or other way around. 3 biological replicates each, total 12 chips.
2007-12-31 | E-TABM-110 | biostudies-arrayexpress
Project description:Nonhost resistance, a resistance of plant species against all nonadapted pathogens, is considered the most durable and efficient immune system in plants. To increase our understanding of the response of barley (Hordeum vulgare L.) plants to infection by powdery mildew, Blumeria graminis f. sp. Tritici, we used quantitative proteomic analysis (LC-MS/MS). We compared the response of two genotypes of barley cultivar Golden Promise, wild type (WT) and plants with overexpression of phytoglobin (previously hemoglobin) class 1 (HO), which has previously been shown to significantly weaken non-host resistance. A total of 8804 proteins were identified and quantified, out of which the abundance of 1044 proteins changed significantly in at least one of the four comparisons (‘i’ stands for ‘inoculated’)- HO/WT and HOi/WTi (giving genotype differences), and WTi/WT and HOi/HO (giving treatment differences). Among these differentially abundant proteins (DAP) were proteins related to structural organization, disease/defense, metabolism, transporters, signal transduction and protein synthesis. More proteins changed in abundance in WT than in HO after inoculation and most DAP increased in abundance.
2020-08-18 | PXD015089 | Pride
Project description:Wild-type barley cv Golden Promise and two independent RNAi knockdown lines (W1-1 And W1-7) of the WHIRLY1 gene were grown under controlled environments. Leaves were harvested after 7 or 14 days and sampled along a developmental gradient of base, mid and tip. Microarray analysis was conducted from three biological replicates using a custom designed Agilent barley microarray. Analysis of transcript abundance suggests developmental delay in WHIRLY knockdown lines with significant disruption of plastid development and transcription.
2021-09-01 | E-MTAB-9882 | biostudies-arrayexpress
Project description:Expression Data of Barley Crown and Growing Point Tissue Under Salt Stress abd JA treatment Experiment Overall Design: Barley genotype Golden Promise was used for expression anlaysis using the tissue from crown and growing point under control, salt stressed, JA treatment and JA pretreatment followed by salt stress
2008-06-13 | E-GEOD-5605 | biostudies-arrayexpress
Project description:Salt Stress response of salt-tolerant genotype Golden Promise compared to Maythorpe Barley1 GeneChip was used to find differential expression between two barley genotypes under control and salt stress conditions at vegetative stage of growth Keywords: genotype and treatment comparison
2007-03-31 | GSE6325 | GEO
Project description:We measured mRNA abundance in the seedling leaves of 8 barley genotypes; Morex, Steptoe, Golden Promise, Optic, Haruna Nijo, Barke, OWB-D and OWB-R. 3 biological replicates each, total 24 chips.
2007-12-31 | E-TABM-113 | biostudies-arrayexpress
Project description:Transcriptional changes were monitored in the barley cultivar Golden Promise 24 hours post inoculation (hpi) with the bacteria Pseudomonas syringae pv. tomato DC3000 avrRpm1 (PstavrRpm1) using the Affymetrix Barley genome array GeneChip®. Seedlings of Golden Promise were grown to growth stage 12-13 (Zadoks et al., 1974) before inoculating with either PstavrRpm1 or water (for the mock inoculation control) by infiltration. Plants were grown under a 18 °C / 16 h light period; 12 °C / 8 h dark period, with artificial lighting (100 µmol m-2 s-1) and a relative humidity of 75 – 85 %. Leaf samples from three seedlings were collected 24 hpi for RNA extraction and transcriptomics analysis from the area infiltrated (local) and from the area next to the infiltrated region (adjacent) from three biological replicates. Leaf tissue was ground under liquid nitrogen and total RNA extracted using the RNeasy miniprep kit (Qiagen), following the manufacturer’s instructions. RNA was DNase treated using Turbo DNase (Ambion) according to the manufacturer instructions. RNA integrity was confirmed using the Agilent 2100 Bioanalyzer (Agilent). The two cycle-target labeling method was used following the Affymetrix protocol. Affymetrix GeneChip processing, including RNA quality control, microarray hybridisation and data acquisition was performed through contract research services by Cogenics (North Carolina, U.S.A.). A total of twelve hybridisations were performed. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Ellen Colebrook. The equivalent experiment is BB92 at PLEXdb.]
2013-01-31 | GSE43906 | GEO