Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The single cell RNA seq of PDGFRa-GFP+ cells in mouse lung

ABSTRACT: The single cell RNA seq of PDGFRa-GFP+ cells in mouse lung

PROVIDER: PRJNA486013 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

The single cell RNA seq of PDGFRa-GFP+ cells in mouse lung

Project description:Pdgfra-expressing (Pdgfra+) cells have been implicated as progenitors in many mesenchymal tissues. To further characterize Pdgfra+ cells during alveologensis, we performed single-cell RNA sequencing (scRNA-Seq) analysis using fluorescence-activated cell sorting (FACS) sorted GFP+ cells from Pdgfra-GFP lungs at P7 and P15.

2018-09-03 | GSE118555 | GEO

Analysis of Nestin-GFP+ pericytes from adipose tissue: PDGFRa wild type versus PDGFRa+/D842V (constitutively active mutant)

Project description:Analysis of Nestin-GFP+ pericytes flow sorted from 3-day-old mouse cutaneous adipose tissue, comparing controls with wild type PDGFRa, and mutants with increased PDGFRa signaling driven by a Cre/lox-inducible D842V knockin mutation in the PDGFRa kinase domain. The control cells have adipogenic properties in vitro or when transplanted subcutaneously into recipient mice. The D842V mutant cells show altered behavior in the same assays, with poor adipogenic differentiation but a propensity to transition into profibrotic cells that secrete collagen

2015-06-01 | GSE64510 | GEO

Cerebral gene expression changes in Pdgfc and Pdgfra mutant

Project description:Platelet-derived growth factor-C (PDGF-C) is one of three known ligands for the tyrosine kinase receptor PDGFRÎ±. Analysis of Pdgfc null mice has demonstrated roles for PDGF-C in palate closure and the formation of cerebral ventricles, but redundancy with other PDGFRÎ± ligands might hide additional functions. In search of further developmental roles for PDGF-C, we generated mice that were double mutants for Pdgfc -/- and Pdgfra GFP/+. These mice display a range of severe phenotypes including cerebellar malformation, neuronal over-migration in the cerebral cortex, spina bifida and lung emphysema. We focused our analysis on the central nervous system (CNS), where PDGF-C was identified as a critical factor for the formation of meninges and assembly of the glia limitans basement membrane. Meninges freely dissected and released from P0 cerebrum. RNA was isolated using RNeasy micro kit (Qiagen) and quality checked in a 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA). Transcription profiling was performed with Gene Chip Mouse Gene 1.0 ST array. We compared Pdgfc-/-; Pdgfra GFP/+ mice, with a control group consisting of all littermates (Pdgfc+/+, Pdgfc+/-, Pdgfc-/-, Pdgfc+/+; Pdgfra GFP/+ and Pdgfc+/-; Pdgfra GFP/+). One litter was used, including 4 mutants and 9 controls

2016-02-24 | E-GEOD-67644 | biostudies-arrayexpress

Single cell RNA-seq of mouse lung endothelial cells

Project description:Single cell RNA sequencing of endothelial cells isolated from adult Prox1-GFP mouse lung

2022-05-11 | GSE197529 | GEO

Single-cell RNA-seq of Pdgfra+/Sca1+/Cd31- mouse cardiac cells

Project description:Adult mice hearts contain a population of resident mesenchymal stem cell (MSC)-like cells called cardiac colony forming units-fibroblast (cCFU-F). The cCFU-F are housed in a population of non-muscle cardiac cells that are Pdgfra+/Sca1+/Cd31- (S+P+ fraction). The goal of this experiment was to profile the heterogeneity of cell sub-types contained within the S+P+ fraction. We profiled two replicates of S+P+ single-cell transcriptomes from the cardiac ventricles of adult, male, C57BL/6J mice after FACS sorting for live Pdgfra+/Sca1+/Cd31- non-myocyte cells.

2019-03-28 | E-MTAB-7365 | biostudies-arrayexpress

Single-cell RNA-seq of Pdgfra+/Sca1+/Cd31- mouse cardiac cells

Project description:Single-cell RNA-seq of Pdgfra+/Sca1+/Cd31- mouse cardiac cells

| PRJEB29524 | ENA

mouse lung single cell RNA-seq

Project description:mouse lung single cell RNA-seq

| PRJNA544965 | ENA

Single-cell transcriptomic analysis of dermal macrophage from PDGFRa-lineage origin under atopic dermatitis

Project description:We analyzed dermal macrophage repertoire derived from PDGFRa-lineage or non-PDGFRa-lineage origin in atopic dermatitis (AD) with single-cell RNA sequencing. PDGFRa-lineage tracing mice aged between 7- and 14-week old were used to label cells of PDGFRa-lineage with tdTomato. AD was induced by topic application of MC903 bidaily or tridaily to skin of female mice for six times. Inflamed skin was excised on day 14 and single cells were prepared. Target cells were sorted as single cells for sequencing. We identified multiple subtypes in both PDGFRa-lineage or non-PDGFRa-lineage macrophage and the proportion differed by PDGFRa-lineage origin in AD skin.

2022-07-27 | GSE166855 | GEO

Cells isolated from diaphragm of mdx mouse: satellite cells vs. PDGFRa+ cells

Project description:Transcriptional profiling of mouse skeletal muscle-derived cells comparing satellite cells with PDGFRa+ cells. Satellite cells and PDGFRa+ cells were directly isolated from diaphragm of dystrophic mdx mouse by FACS. Two-condition experiment, satellite cells vs. PDGFRa+ cells. Freshly isolated. One replicate per array.

2011-12-08 | E-GEOD-25258 | biostudies-arrayexpress

Cells isolated from diaphragm of mdx mouse: satellite cells vs. PDGFRa+ cells

2011-12-08 | GSE25258 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data