Project description:To identify CdbA binding sites on Myxococcus xanthus genome in vivo we performed ChIP-seq, using a polyclonal anti-FLAG antibody and a strain endogenously expressing CdbA_3xFLAG. A WT DK1622 strain was used a negative control and a strain endogenously expressing ParB_3xFLAG was used as positive control.
Project description:In response to starvation Myxococcus xanthus initiates a developmental program that culminates in the formation of fruiting bodies inside which the rod-shaped cells differentiate to spores. Fruiting body formation depends on intercellular communication and two intercellular signals are known, the A-signal and the C-signal. Five genes have been identified which are required for A-signal synthesis. To begin to understand the function of the genes required for A-signal synthesis, we have analysed gene expression in the asgA and the asgB mutant. Keywords: Vegetative cells of WT (DK1622) and AsgA mutant (DK5057) and AsgB mutant (DK4398)
Project description:In response to starvation Myxococcus xanthus initiates a developmental program that culminates in the formation of fruiting bodies inside which the rod-shaped cells differentiate to spores. Fruiting body formation depends on intercellular communication and two intercellular signals are known, the A-signal and the C-signal. Five genes have been identified which are required for A-signal synthesis. To begin to understand the function of the genes required for A-signal synthesis, we have analysed gene expression in the asgA and the asgB mutant. Keywords: Vegetative cells of WT (DK1622) and AsgA mutant (DK5057) and AsgB mutant (DK4398) 3 biological replicates each; normalized ratios to vegetative cells of DK1622 (wt) Cy5
Project description:In Myxococcus xanthus 55% of the more than 250 two-component signal transduction systems (TCS) genes are orphan. We hypothesized that the histidine kinase SgmT and the response regulator DigR, which comprises a DNA binding domain of the HTH_Xer type, function together to regulate gene expression. We performed genome-wide expression profiling experiments to determine wether the same set of genes are differentially expressed in the ΔdigR and ΔsgmT mutants. 3 biological replicates each; normalized ratios to vegetative cells of DK1622 (wt) Cy5
Project description:In response to starvation, Myxococcus xanthus initiates a developmental program that results in the formation of spore-filled multicellular fruiting bodies. Here we have used cDNA microarray analysis to determine changes in the global gene expression at different time points of the developmental process. The expression of nearly 900 genes was found to be altered at least two-fold during development as compared to vegetative cells. Genes encoding proteins with typical vegetative functions such as protein synthesis and energy metabolism were transcriptionally down-regulated in the early stages of development. Among the 430 genes transcriptionally up-regulated during development genes with regulatory functions were overrepresented; underlining that fruiting body formation relies on a complex signalling network. Notably, almost 40% of all genes with increased expression at different stages of development encoded hypothetical proteins indicating a large unexplored potential of proteins important for fruiting body formation. Keywords: Time course of development with 9 time points 3 biological replicates each; normalized ratios to vegetative cells of DK1622 (wt) Cy5
