Project description:Chinese Chestnut (Castanea mollissima BL.) has high nutritional and ecological value, so it is widely planted.However, the number of male flowers far exceeds the number of female flowers is an important factor limiting the yield of Chinese chestnut.A naturally occurring mutation in male catkin has been found on a Chinese chestnut tree in the mountains of Beijing, China.This mutant is shorter than the normal catkin.Studies have shown that this mutation can significantly reduce the number of male flowers, promote the differentiation of female flowers, and improve the yield of Chinese chestnut.In this research, we provided the RNA sequencing data of two groups of mutants and normal catkin.The comparative analyses of catkins transcriptomes presented here provides a valuable resource for discovery of genes and networks involved in the regulate the sex of the flower.
Project description:In this study, the Chinese chestnut ‘Huaihuang’ was used to explore the possible mechanisms of ovule abortion with respect to proteomics. The chestnut anthesis starts mid-June. The development of the burs of C. mollissima cv. ‘Huaihuang’ were monitored from 15 to 25 days after anthesis (DAA) And the burs for different times were collected from the Chestnut Experiment Station in Huairou District, Beijing, China. This experiment was conducted at the Beijing Protein Innovation Co., Ltd.
Project description:DNA methylation analysis using the Infinium HumanMethylation450 BeadChip platform (Illumina) of 96 Caucasian American, 96 Han Chinese American and 96 African American LCL samples determined differences in terms of differentially methylated sites. Importantly, the observed differences were confirmed in primary blood samples of 10 healthy Caucasian, 10 African American (GSE36064) and 10 Asian individuals. Genes associated to differentially methylated site suggest an influence of DNA methylation on phenotype differences. Interestingly, methylation differences could be partially traced back to genetic polymorphisms.
