Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Unbiased screen of RNA tailing enzymes reveals a poly(UG) polymerase required for genome integrity and RNA silencing

ABSTRACT: Unbiased screen of RNA tailing enzymes reveals a poly(UG) polymerase required for genome integrity and RNA silencing

PROVIDER: PRJNA508883 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Unbiased screen of RNA tailing enzymes reveals a poly(UG) polymerase required for genome integrity and RNA silencing

Project description:Ribonucleotidyl transferases (rNTases) add non-templated ribonucleotides to diverse RNAs. We developed a screening strategy in S. cerevisiae to identify sequences added by candidate enzymes from different organisms at single-nucleotide resolution. The rNTase activities of 19 previously unexplored enzymes were determined. In addition to poly(A)- and poly(U)-adding enzymes, we identified a C-adding enzyme that is likely part of a two-enzyme system that adds CCA to tRNAs in a eukaryote; a nucleotidyl transferase that adds nucleotides to RNA without apparent nucleotide preference; and a poly(UG) polymerase, C. elegans MUT-2, which adds alternating U and G nucleotides to form poly(UG) tails. MUT-2 is known to be required for certain forms of RNA silencing, and mutations in the enzyme that are defective in silencing also fail to add poly(UG) tails in our assay. We propose that MUT-2 poly(UG) polymerase activity is required to promote genome integrity and RNA silencing.

2018-12-08 | GSE123478 | GEO

Unbiased screen of RNA tailing activities reveals a poly(UG) polymerase.

Project description: Not available

| S-EPMC6613791 | biostudies-literature

Impact of URT1-mediated uridylation on small RNA tailing in Arabidopsis

Project description:To analyse the impact of URT1-mediated uridylation on miRNA and siRNA tailing, we deep-sequenced small RNA libraries for WT and urt1 duplicate samples at the same developmental stage that was analyzed by TAIL-seq, i.e., two-week-old seedlings. Examination of miRNA and siRNA tailing in WT and urt1 samples.

2016-03-11 | E-GEOD-72456 | biostudies-arrayexpress

poly(UG)-tailed RNAs in Genome Protection and Epigenetic Inheritance [RNA-Seq]

Project description:poly(UG)-tailed RNAs in Genome Protection and Epigenetic Inheritance [RNA-Seq]

| PRJNA623004 | ENA

poly(UG)-tailed RNAs in Genome Protection and Epigenetic Inheritance

Project description:poly(UG)-tailed RNAs in Genome Protection and Epigenetic Inheritance

| PRJNA623321 | ENA

tailing ponds sediment

Project description:tailing ponds sediment Raw sequence reads

| PRJNA431952 | ENA

Study of TUT tailing on miRNA

Project description:Many microRNAs (miRNAs) exist alongside abundant miRNA isoforms (isomiRs), most of which arise from post-maturation sequence modifications, such as 3’ uridylation and adenylation. However, the ways in which these sequence modifications affect miRNA function remain poorly understood. To this aim, we have generated single knock-out cell lines of TUT4, TUT7 and TENT2 (TUT2), as well as double knock-out (DKO) and triple knock-out (TKO) cell lines. Here, using these different cell lines, we have discovered that some of the redundant functions and specific functions of each tailing enzyme. Our study provides a comprehensive characterization of tailing on miRNAs.

2022-08-15 | GSE183384 | GEO

Copper mine tailing metagenome

Project description:Copper mine tailing metagenome Metagenome

| PRJNA474760 | ENA

Impact of URT1-mediated uridylation on small RNA tailing in Arabidopsis

2016-03-11 | GSE72456 | GEO

poly(UG)-tailed RNAs in Genome Protection and Epigenetic Inheritance [other]

Project description:poly(UG)-tailed RNAs in Genome Protection and Epigenetic Inheritance [other]

| PRJNA623329 | ENA