Project description:RCH-ACV cells was depleted for SETDB2 by shRNA lentivirus construct co-expressing mCherry fluorescent. After sorting of mCherry positive cells, the cells were cultured and expanded and total RNA was extracted for Microarray experiment.
Project description:Mouse IMCD3 cell lines knocked-down for VPS33B, VIPAR and PLOD3 are used as models of ARC syndrome, an autosomal recessive disorder cause by mutations in VPS33B or VIPAR. Previous work has shown that ARC syndrome causes epithelial cell polarisation defectes and mislocalisation of membrane proteins, so polarised IMCD3 cells were used as an experimental model. Affymetrix arrays were used to characterise the changes to the transcriptome when protein levels of VPS33B, VIPAR and PLOD3 are reduced. mouse IMCD-3 cell lines, 15 samples in total, three biological repeats of each of: wild type, control shRNA, VPS33B shRNA, VIPAR shRNA, PLOD3 shRNA
Project description:The purpose of the study is to understand the impacts of cohesin knockdown on epidermal gene expression. Primary human keratinocytes were knocked down for SMC1A, SMC3, or control to determine its genome-wide gene expression profile. Global gene expression profiling using U133 plus 2.0 arrays were used to deteremine the genes perturbed by knockdown of SMC1A or SMC3.