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Chaetocin Mediated Changes in Gene Expression Profile of GBM Cells

ABSTRACT: Chaetocin Mediated Changes in Gene Expression Profile of GBM Cells

PROVIDER: PRJNA521979 | ENA |

REPOSITORIES: ENA

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Chaetocin Mediated Changes in Gene Expression Profile of GBM Cells

Project description:Chaetocin treatment significantly modulated expression profile of GBM cells such that 373 genes were up and 478 genes were down regulated significantly (FDR<0.05). Genes involved in DNA damage response, reactive oxygen species pathway and cell cycle regulation as well as epithelial to mesenchymal transition are significantly modified by Chaetocin, which render GBM cells much more prone to apoptosis.

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Human GBM tumor vs Normal Human DNA

Project description:We have developed a nonheuristic genome topography scan (GTS) algorithm to characterize the patterns of genomic alterations in human glioblastoma (GBM), identifying frequent p18INK4C and p16INK4A codeletion. Functional reconstitution of p18INK4C in GBM cells null for both p16INK4A and p18INK4C resulted in impaired cell-cycle progression and tumorigenic potential. Conversely, RNAi-mediated depletion of p18INK4C in p16INK4A-deficient primary astrocytes or established GBM cells enhanced tumorigenicity in vitro and in vivo. Furthermore, acute suppression of p16INK4A in primary astrocytes induced a concomitant increase in p18INK4C. Together, these findings uncover a feedback regulatory circuit in the astrocytic lineage and demonstrate a bona fide tumor suppressor role for p18INK4C in human GBM wherein it functions cooperatively with other INK4 family members to constrain inappropriate proliferation. Keywords: comparative genomic hybridization DNA copy number abberation of human glioblastoma tumors were obtained by comparative genomic hybridization of GBM tumor vs. normal human DNA. 11 human GBM samples were analyzed on Agilent human 244A human cgh array (G4411B). Normal Human DNA was used as reference. Some samples were hybridized with dye-swap replica.

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