Project description:To identify how mu-crystallin operates within striatal astrocytes, we used proximity-dependent biotinylation with BioID2 to identify mu-crystallin interacting proteins relative to GFP controls. Strong biotinylation in vivo permitted identification of the 78-protein mu-crystallin interactome, which will be fully reported in a following publication
Project description:Mu killer contains a partial inverted duplication of the mudrA transposase gene and two copies of the terminal inverted repeat A region of MuDR. Mu killer can effectively silence single copy MuDR/Mu lines but failed to silence about 25% of the time in multiple copy lines. Mu killer was partially sequenced and shown to be collinear with mudrA. Mu killer individuals that silenced MuDR contained two short antisense transcripts, while individuals that failed to silence MuDR contained multiple sense transcripts but no antisense transcripts. Transcriptomes from Mu killer-silenced individuals were compared to epigenetically silenced lines; the 2 silencing mechanisms were shown to affect different pathways.